Isolation of genes expressed asymmetrically in flounder metamorphosis and regulation by thyroid hormone

• Project/Area Number: 15K07571
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Tohoku University
• Principal Investigator: Yokoi Hayato 東北大学, 農学研究科, 助教 (40569729)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ヒラメ / 変態 / 異体類 / 次世代シーケンサー / 次世代シーケンス解析 / 発生

Outline of Final Research Achievements

Flounder transform their body plan during metamorphosis; right eye migrates to the other side and pigment cells differentiate only in the ocular side. Gene expression profile were compared between ocular and blind side in tissues around supra orbital bar at early metamorphic stage and skin and muscle tissue at later metamorphic stage in order to isolate genes expressed asymmetrically between ocular and blind side. Candidate genes were further analyzed by in situ hybridization and suggested that genes might be involved in right side specific degeneration of supra orbital bar and genes that genes might be involved in ocular side specific pigment cell differentiation.

Research Products

• [Int'l Joint Research] ハイデルベルク大学医学部マンハイム校(ドイツ)
• [Int'l Joint Research] バルセロナ大学遺伝学部門/国立海洋研究所CSIC(スペイン)
• [Journal Article] Analyses of the cellular clock gene expression in peripheral tissue, caudal fin, in the Japanese flounder, Paralichthys olivaceus (2017)
  • Author(s): M. Mogi, S. Yokoi, T. Suzuki,
  • Journal Title: General and Comparative Endocrinology Volume: 印刷中 Pages: 97-105
  • DOI: 10.1016/j.ygcen.2017.02.009
  • Peer Reviewed
• [Journal Article] External Asymmetry and Pectoral Fin Loss in the Bamboo Sole (Heteromycteris japonica): Small-Sized Sole with Potential as a Pleuronectiformes Experimental Model (2017)
  • Author(s): Chen Q, Takagi M, Mogi M, Kikuchi M, Saito Y, Nakamura S, Yokoi H, Seikai T, Uji S and Suzuki T
  • Journal Title: Zoolog Sci Volume: 34 Issue: 5 Pages: 377-385
  • DOI: 10.2108/zs170021
  • NAID: 40021336139
  • Peer Reviewed
• [Journal Article] Excess retinoic acid induces fusion of centra by degenerating intervertebral ligament cells in Japanese flounder, Paralichthys olivaceus (2017)
  • Author(s): Wu X, Chen Q, Washio Y, Yokoi H, Suzuki T
  • Journal Title: Journal of Experimental Zoology, Part B, Molecular and Developmental Evolution Volume: 326B Issue: 8 Pages: 464-473
  • DOI: 10.1002/jez.b.22717
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Diethylnitrosamine-induced expression of germline-specific genes and pluripotency factors, including vasa and oct4, in medaka somatic cells. (2016)
  • Author(s): Shen J, Yokota S, Yokoi H and Suzuki T
  • Journal Title: Biochem Biophys Res Commun Volume: 478 Issue: 2 Pages: 858-863
  • DOI: 10.1016/j.bbrc.2016.08.039
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Role of notochord cells and sclerotome derived cells in vertebral column development in fugu, Takifugu rubripes: histological and gene expression analyses (2016)
  • Author(s): T. Kaneko, K. Freeha, X. Wu, M. Mogi, S. Uji, H. Yokoi, T. Suzuki,
  • Journal Title: Source of the Document Cell and Tissue Research Volume: 366 Issue: 1 Pages: 37-49
  • DOI: 10.1007/s00441-016-2404-z
  • Peer Reviewed
• [Journal Article] Early development of circadian rhythmicity in the suprachiamatic nuclei and pineal gland of teleost, flounder (Paralichthys olivaeus), embryos. (2015)
  • Author(s): Mogi M, Uji S, Yokoi H and Suzuki T
  • Journal Title: Dev Growth Differ Volume: 57 Issue: 6 Pages: 444-452
  • DOI: 10.1111/dgd.12222
  • Peer Reviewed
• [Journal Article] Transcriptome analysis of vertebral bone in the flounder, Paralichthys olivaceus (Teleostei, Pleuronectiformes), using Illumina sequencing. (2015)
  • Author(s): Ibaraki H, Wu X, Uji S, Yokoi H, Sakai Y and Suzuki T
  • Journal Title: Mar Genomics Volume: 24 Pages: 269-276
  • DOI: 10.1016/j.margen.2015.09.009
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 変態期ヒラメの RNA-seq 解析で単離された色素胞分化に関与する遺伝子の解析 (2018)
  • Author(s): 横井勇人、Rotllant Josep、Cal Laura、鈴木徹
  • Organizer: 日本水産学会春季大会
• [Presentation] 魚類における視交叉上核を介した体内時計の制御機構に関する研究 (2018)
  • Author(s): 茂木淳、横井勇人、鈴木徹
  • Organizer: 日本水産学会春季大会
• [Presentation] ヒラメの着色型黒化部に現れる黒色素胞の起源について (2018)
  • Author(s): 十川麻衣、松田直往、田川正朋、横井勇人、鈴木徹
  • Organizer: 日本水産学会春季大会
• [Presentation] ヒラメ下垂体における時計遺伝子と内分泌関連遺伝子の概日リズムに関する解析 (2018)
  • Author(s): 丹羽祥太、茂木淳、横井勇人、鈴木徹
  • Organizer: 日本水産学会春季大会
• [Presentation] ヒラメ末梢組織における時計遺伝子発現の日周リズムの観察とそれらに及ぼすコルチゾル添加の影響の解析 (2017)
  • Author(s): 茂木淳、横井勇人、鈴木徹
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都・港区）
  • Year and Date: 2017-03-26
• [Presentation] 給餌条件により生じるヒラメ体色異常の遺伝子発現プロファイリング解析 (2017)
  • Author(s): 國政実里、横井勇人、酒井義文、青海忠久、鈴木徹
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都・港区）
  • Year and Date: 2017-03-26
• [Presentation] ヒラメ再生皮膚に現れる色素前駆細胞と色素胞の分布 (2017)
  • Author(s): 十川麻衣、茂木淳、横井勇人、鈴木徹
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都・港区）
  • Year and Date: 2017-03-26
• [Presentation] エレクトロポレーション法による成体へのプラスミド導入とタンパク合成誘導効果の検討 (2017)
  • Author(s): 岩泉雅樹、横井勇人、鈴木徹
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都・港区）
  • Year and Date: 2017-03-26
• [Presentation] Expression profiling of Japanese flounder abnormal pigmentation caused by feeding conditions (2017)
  • Author(s): Kunimasa M, ○Yokoi H, Sakai Y, Seikai T and Susuki T
  • Organizer: 50th annual meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)
  • Int'l Joint Research
• [Presentation] Small-scale culture system of bamboo sole (Heteromycteris japonica), a small-sized species of the Pleuroneciformes, and its metamorphic external asymmetry and pectoral fin loss (2017)
  • Author(s): Suzuki T, Chen Q, Uji S and Yokoi H
  • Organizer: 50th annual meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)
  • Int'l Joint Research
• [Presentation] RHYTHMIC PER2 EXPRESSION AT THE SUPRACHIASMATIC NUCLEUS OF THE JAPANESE FLOUNDER, PARALICHYTHYS OLIVACEUS, AND ITS IMPLICATIONS FOR CIRCADIAN CLOCK MECHANISM (2017)
  • Author(s): Suzuki T, Mogi M and Yokoi H
  • Organizer: 18th International Congress of Comparative Endocrinology (ICCE18)
  • Int'l Joint Research / Invited
• [Presentation] EXPRESSION OF THREE CLOCK GENES, PER1, PER2 AND CRY1 IN THE CAUDAL FIN OF JAPANESE FLOUNDER (2017)
  • Author(s): Mogi M, Yokoi H and Suzuki T
  • Organizer: 18th International Congress of Comparative Endocrinology (ICCE18)
  • Int'l Joint Research / Invited
• [Presentation] Transcriptome analysis of flatfish metamorphosis identified ocular side biased genes involved in pigmentation (2017)
  • Author(s): Yokoi H, Kunimasa M, Sakai Y and Suzuki T
  • Organizer: 10th European Zebrafish Meeting
  • Int'l Joint Research
• [Presentation] Whole genome duplication and diversification of gene function in teleosts: lessons from Fgfr1, Sox9 and Aldh genes (2017)
  • Author(s): Yokoi H, Qiran C, Canestro C, Postlethwait JH and Suzuki T
  • Organizer: XI Congress of Iberian Association for Comparative Endocrinology (AIEC)
  • Int'l Joint Research / Invited
• [Presentation] ヒラメ変態期の左右非対称な形態形成と細胞分化 (2017)
  • Author(s): 横井勇人、鈴木徹
  • Organizer: 第3回ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会
• [Presentation] Expression profile of RA-related genes during metamorphic development in Japanese flounder (2017)
  • Author(s): Chen Q, Yokoi H and Suzuki T
  • Organizer: 23rd Japanese Medaka and Zebrafish Meeting
  • Int'l Joint Research
• [Presentation] Application of electroporation to zebrafish and bubble-eye goldfish for exogenous protein synthesis in the ovary and skeletal muscle (2017)
  • Author(s): Iwaizumi M, Yokoi H and Suzuki T
  • Organizer: 23rd Japanese Medaka and Zebrafish Meeting
  • Int'l Joint Research
• [Presentation] ヒラメの無眼側皮膚に異所的に出現する色素胞の由来について (2017)
  • Author(s): 十川 麻衣、茂木淳、横井勇人、鈴木徹
  • Organizer: 日本動物学会 第88回 富山大会
• [Presentation] トラフグの脊椎骨で椎間板様組織を構成する脊索のトランスクリプトーム解析 (2017)
  • Author(s): 鈴木徹、明珍大志、横井勇人
  • Organizer: 日本動物学会 第88回 富山大会
• [Presentation] Pigmentation genes isolated by transcriptome expression profiling of flounder metamorphosis (2017)
  • Author(s): Yokoi H, Cal L, Rotllant J and Susuki T
  • Organizer: 2017年度生命科学系学会合同年次大会、第40回日本分子生物学会年会、第90回日本生化学会大会
• [Presentation] Expression profiling of flatfish, a weird teleost fish that transforms into an asymmetric body plan (2016)
  • Author(s): Yokoi H, Kunimasa M, Washio Y, Wu X, Sakai Y Seikai T and Suzuki T
  • Organizer: The joint meeting of the 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society of Japan
  • Place of Presentation: Okinawa Convention Center (Okinawa, Ginowan)
  • Year and Date: 2016-11-14
  • Int'l Joint Research / Invited
• [Presentation] Rhythmicity of clock genes, per2, per1 and cry1, in peripheral tissue in flounder (Paralichthys olivaceus), a marine teleost fish (2016)
  • Author(s): Mogi M, Yokoi H and Suzuki T
  • Organizer: The joint meeting of the 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society of Japan
  • Place of Presentation: Okinawa Convention Center (Okinawa, Ginowan)
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Embryonic development of circadian rhythmicity in the suprachiasmatic nucleus and pineal gland in flounder (Paralichthys olivaceus), a teleost fish (2016)
  • Author(s): Mogi M, Yokoi H and Suzuki T
  • Organizer: International Symposium on Biological Rhythms “Towards understanding the molecular clockwork”
  • Place of Presentation: Toyoda auditorium, Nagoya University (Aichi, Nagoya)
  • Year and Date: 2016-11-11
  • Int'l Joint Research
• [Presentation] 次世代シーケンサーを用いた遺伝子発現プロファイリングによるヒラメの体色異常に関与する遺伝子の解析 (2016)
  • Author(s): 國政実里、横井勇人、青海忠久、鈴木徹
  • Organizer: 日本水産学会北海道-東北合同支部大会
  • Place of Presentation: 北海道大学水産科学研究院（北海道・函館市）
  • Year and Date: 2016-10-22
• [Presentation] Expression of RA synthases, aldh1a1, 1a2 and 1a3, in newly hatched, metamorphic larvae and adult of Japanese founder (2016)
  • Author(s): 陳其然、横井勇人、鈴木徹
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 近畿大学農学部（奈良県・奈良市）
  • Year and Date: 2016-09-08
• [Presentation] ヒラメの着色型黒化進行部位における色素前駆細胞の分布 (2016)
  • Author(s): 十川麻衣、茂木淳、横井勇人、鈴木徹
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 近畿大学農学部（奈良県・奈良市）
  • Year and Date: 2016-09-08
• [Presentation] ブラジル産とユタ産アルテミアを与えたヒラメ変態期仔魚で発現量の異なる遺伝子のRNA-Seqによるスクリーニング (2016)
  • Author(s): 國政実里、青海忠久、鈴木徹、横井勇人
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 近畿大学農学部（奈良県・奈良市）
  • Year and Date: 2016-09-08
• [Presentation] DENによるメダカ再生鰭でのvasa, nanosの発現誘導 (2016)
  • Author(s): Jialing Shen、横井勇人、鈴木徹
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 近畿大学農学部（奈良県・奈良市）
  • Year and Date: 2016-09-08
• [Presentation] TALEN 法を用いて作製した r-spondin2 変異体ゼブラフィッシュの表現型 (2016)
  • Author(s): 岩泉雅樹、辰見吉昭、武田萌、鈴木徹、横井勇人
  • Organizer: 日本ゲノム編集学会第1回大会
  • Place of Presentation: 広島国際会議場（広島県・広島市）
  • Year and Date: 2016-09-06
• [Presentation] ヒラメ：左右非対称な眼の移動と色素胞分化 (2016)
  • Author(s): 横井勇人
  • Organizer: 第２回ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県・岡崎市）
  • Year and Date: 2016-08-21
  • Invited
• [Presentation] Transcriptome analysis of flounder metamorphosis (2016)
  • Author(s): Yokoi H, Kunimasa M, Sakai Y and Suzuki T
  • Organizer: 22nd Japanese medaka and zebrafish meeting
  • Place of Presentation: Okazaki Conference Center (Aichi, Okazaki)
  • Year and Date: 2016-08-20
  • Int'l Joint Research
• [Presentation] 変態期ヒラメの眼球移動に関与する遺伝子の探索 (2016)
  • Author(s): 横井勇人、酒井義文、鈴木徹
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都・港区）
  • Year and Date: 2016-03-26
• [Presentation] ヒラメにおける鱗の発生と色素胞、色素前駆細胞の挙動 (2016)
  • Author(s): 十川麻衣、茂木淳、横井勇人、鈴木徹
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都・港区）
  • Year and Date: 2016-03-26
• [Presentation] Vitamine A metabolism, revealed by RNA-seq, in the notochord constituting intervertebral tissue (2016)
  • Author(s): Freeha K, Yokoi H and Suzuki T
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都・港区）
  • Year and Date: 2016-03-26
• [Presentation] ヒラメにおける時計遺伝子の日周リズムおよび光同調性の解析 (2016)
  • Author(s): 茂木淳、横井勇人、鈴木徹
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都・港区）
  • Year and Date: 2016-03-26
• [Presentation] エレクトロポレーションによる成体器官への遺伝子導入効果の検討 (2016)
  • Author(s): 岩泉雅樹、横井勇人、鈴木徹
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都・港区）
  • Year and Date: 2016-03-26
• [Presentation] Induction of germline-specic and pluripotency factor mRNAs, including vasa and oct4, in medaka somatic cells by diethylnitrosamine (2016)
  • Author(s): Shen J, Yokoi H and Suzuki T
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都・港区）
  • Year and Date: 2016-03-26
• [Presentation] Photoentrainment of dairy rhythmicity in the SCN and peripheral tissue in teleost fish, Japanese flounder (Paralichthys olivaceus) (2016)
  • Author(s): 茂木淳、横井勇人、鈴木徹
  • Organizer: 第23回日本時間生物学会学術大会
  • Place of Presentation: 名古屋大学豊田講堂（愛知県・名古屋市）
• [Presentation] ヒラメを用いた魚類概日リズムの光同調機構の解析 (2015)
  • Author(s): 茂木淳、宇治督、横井勇人、鈴木徹
  • Organizer: BMB2015(第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸ポートアイランド（兵庫県・神戸市）
  • Year and Date: 2015-12-01
• [Presentation] 変態期ヒラメ頭部で左右非対称に発現する遺伝子の探索 (2015)
  • Author(s): 横井勇人、久保田直喜、烏暁明、酒井義文、鈴木徹
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 東北大学川内キャンパス（宮城県・仙台市）
  • Year and Date: 2015-09-22
• [Presentation] ヒラメにおける視交叉上核、松果体および体細胞の概日リズムの発生 (2015)
  • Author(s): 茂木淳、宇治督、横井勇人、鈴木徹
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 東北大学川内キャンパス（宮城県・仙台市）
  • Year and Date: 2015-09-22
• [Presentation] Generation of tumor protein p53, tp53, Knockout Medaka Using an oct4-gfp/β-actin-dsred Double Transgenic Line (2015)
  • Author(s): Shen J, Yokota S, Kato H, Kinoshita M, Yokoi H and Suzuki T
  • Organizer: 21st Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: Ichokaikan, Osaka University (Osaka, Suita city)
  • Year and Date: 2015-09-19
  • Int'l Joint Research
• [Presentation] ヒラメ・カレイ類の左右非対称な体色形成と着色異常の発生機構 (2015)
  • Author(s): 鈴木徹、鷲尾洋平、烏暁明、國政実里、十川麻衣、横井勇人
  • Organizer: 日本動物学会 第11回色素細胞シンポジウム:動物のからだを多様に彩るしくみ
  • Place of Presentation: 朱鷺メッセ 新潟コンベンションセンター（新潟県・新潟市）
  • Year and Date: 2015-09-17
  • Invited
• [Presentation] 変態期ヒラメの左右非対称化の分子基盤を解明したい：RNA seqによるアプローチ (2015)
  • Author(s): 横井勇人
  • Organizer: ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県・岡崎市）
  • Year and Date: 2015-08-18
  • Invited
• [Presentation] 左ヒラメと右カレイを分ける発生システム (2015)
  • Author(s): 鷲尾洋平、横井勇人、鈴木徹
  • Organizer: 日本動物学会 平成27年度東北支部会
  • Place of Presentation: 東北大学農学研究科（宮城県・仙台市）
  • Year and Date: 2015-08-08
• [Presentation] Transcriptome analysis of asymmetric pigmentation in the flounder metamorphosis (2015)
  • Author(s): Yokoi H, Kunimasa M, Wu X, Sakai Y and Suzuki T
  • Organizer: 48th annual meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)
  • Place of Presentation: Tsukuba International Congress Center (Ibaraki, Tsukuba)
  • Year and Date: 2015-06-02
  • Int'l Joint Research
• [Presentation] Post-metamorphic ectopic pigmentation at the ocular side skin in flatfish (2015)
  • Author(s): Suzuki T, Kunimasa M, Wu X, Togawa M, Uji S and Yokoi H
  • Organizer: 48th annual meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)
  • Place of Presentation: Tsukuba International Congress Center (Ibaraki, Tsukuba)
  • Year and Date: 2015-06-02
  • Int'l Joint Research
• [Presentation] Effects of light conditions on the development of circadian rhythm in the suprachiasmatic nuclei and pineal in embryos of a marine fish, flounder (Paralichthys olivaceus) (2015)
  • Author(s): Mogi M, Uji S, Yokoi H and Suzuki T
  • Organizer: 48th annual meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)
  • Place of Presentation: Tsukuba International Congress Center (Ibaraki, Tsukuba)
  • Year and Date: 2015-06-02
  • Int'l Joint Research
• [Book] Whole genome duplication and diversification of gene function in teleosts: lessons from Fgfr1, Sox9 and Aldh genes. In "Advances in Comparative Endocrinology, vol IX". (2017)
  • Author(s): Yokoi H, Chen Q, Canestro C, Postlethwait JH, Suzuki T
  • Publisher: Universida de Vigo
  • ISBN: 9788481587586
• [Remarks] 東北大学研究者紹介
  • URL: http://db.tohoku.ac.jp/whois/detail/0b42e27653b7f35e08dfd12bc8aceb84.html