| Project/Area Number |
15K07708
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Animal production science
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | CD36 / 脂肪滴含有筋線維 / 放牧 / 日本短角種 / 筋線維型構成割合 / 筋線維内脂肪滴 / 筋線維型 / 組織・細胞 / 自給飼料 / 赤身牛肉 |
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| Outline of Final Research Achievements |
The purpose of this study is to investigate the effects of grazing on lipid droplets accumulation in skeletal muscle of Japanese Shorthorn cattle. We analyzed distribution of muscle fiber types and intramyocellular lipid, identification of CD36 immunoreactivity myofiber, expression of myogenic (MyoD, myogenin, myostatin, ActR2B) and lipogenic (DGAT1 and 2, LPL, HSL) genes of M. biceps femoris (BF). Serum NEFA concentrate increased among 0-60 days after grazing. CD36 immunoreactivity myofiber was found in about 20% of BF. Expression of myogenic genes were no significant difference among grazed and housed managements. We found that lipogenic genes of DGAT2, LPL, HSL increase on grazing. It suggests that myogenic genes may not contribute to lipid droplets accumulation in BF. These findings can provide a basis of the complete grass-fed beef production system, furthermore, lipid droplets accumulation by grazing, the mechanism was partly revealed to be through CD36.
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