Comprehensive detection methods for mosquito-borne viruses
| Project/Area Number |
15K07715
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Hokkaido University |
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Principal Investigator |
OBA YASUKO 北海道大学, 人獣共通感染症リサーチセンター, 講師 (60507169)
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 感染症 / 蚊媒介性ウイルス / アルボウイルス / 診断法 / 蚊媒介性 / RT-PCR / ウイルス |
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| Outline of Final Research Achievements |
In order to identify arboviruses in mosquitoes, we have established the one-step RT-PCR methods to detect broad arboviruses by using degenerated primers against flaviviruses, alphaviruses, phleboviruses respectively. Using these comprehensive methods, we have identified many mosquito-borne viruses including previously unidentified viruses and West Nile virus from field-collected mosquitoes in Zambia. These methods were applied to the detection of arboviruses in samples from febrile human cases in Zambia.
Our results using established methods provide the first evidence of the circulation of West Nile virus in Zambia and suggest there should be an increased awareness of possible associated human and animal diseases in that country.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で確立したアルボウイルスの遺伝子検査系を用いて、感染症の先回り対策として、アフリカのザンビア共和国において蚊におけるアルボウイルスのスクリーニングを実施した。その結果、ザンビアで初めてウエストナイルウイルスを検出し、臨床上重要なアルボウイルスの存在を明らかにした。ザンビアではヒトでのウエストナイル熱を含むアルボウイルス感染症発生の報告がないことから、この結果をザンビアの保健省等に報告し、感染症流行予防の対策を講じる上で重要な成果となった。熱性疾患の患者由来検体のアルボウイルス遺伝子検査にも確立した方法を応用した。
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Report
(5 results)
Research Products
(19 results)
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[Journal Article] First isolation of West Nile virus in Zambia from mosquitoes.2018
Author(s)
Orba Y, Hangombe BM, Mweene AS, Wada Y, Anindita PD, Phongphaew W, Qiu Y, Kajihara M, Mori-Kajihara A, Eto Y, Sasaki M, Hall WW, Eshita Y, Sawa H.
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Journal Title
Transboundry Emerging Disease
Volume: 65(4)
Issue: 4
Pages: 933-938
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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