| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
In order to identify arboviruses in mosquitoes, we have established the one-step RT-PCR methods to detect broad arboviruses by using degenerated primers against flaviviruses, alphaviruses, phleboviruses respectively. Using these comprehensive methods, we have identified many mosquito-borne viruses including previously unidentified viruses and West Nile virus from field-collected mosquitoes in Zambia. These methods were applied to the detection of arboviruses in samples from febrile human cases in Zambia.
Our results using established methods provide the first evidence of the circulation of West Nile virus in Zambia and suggest there should be an increased awareness of possible associated human and animal diseases in that country.
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