Improved safety and immunogenicity of rabies live vaccine by a reverse genetics approach
| Project/Area Number |
15K07720
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Gifu University |
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Principal Investigator |
ITO Naoto 岐阜大学, 応用生物科学部, 准教授 (20334922)
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| Research Collaborator |
OKADA Kazuma
NAKAGAWA Kento
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 狂犬病ワクチン / 狂犬病ウイルス / 生ワクチン / 遺伝子改変 / 狂犬病 / ワクチン / 遺伝子操作 |
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| Outline of Final Research Achievements |
In this study, by using a reverse genetics system of the live rabies vaccine strain ERA, we aimed to improve safety and immunogenicity of live rabies vaccine. By introducing a novel attenuating mutation into the viral genome, we have successfully established a live vaccine candidate that is highly and stably attenuated. Meanwhile, although we have generated a candidate with codon-optimized G gene, we were not able to observe increased expression level of G protein and enhanced immunogenicity. The findings above will be important fundamental information for development of live rabies vaccines by using a reverse genetics approach in the future.
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Report
(4 results)
Research Products
(3 results)
