| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We demonstrated the presence of wild SAA and the mutant SAA in deposited amyloid in AA amyloidosis of dog, cat, cow and goat by mass spectrometry. An in vitro fibrillation assay demonstrated that the mutant SAA peptide formed typically rigid, long amyloid fibrils (10 nm wide), and the wild SAA peptide formed zigzagged, short fibers (7 nm wide). Moreover, the wild SAA peptide formed long, rigid amyloid fibrils in the presence of sonicated amyloid fibrils formed by the mutant SAA peptide. These findings indicate that the mutant SAA and wild SAA peptide mediate development of AA amyloidosis in animals via cross-seeding polymerization.
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