| Project/Area Number |
15K07734
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Oka Tomoichiro 国立感染症研究所, ウイルス第二部, 主任研究官 (50356242)
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| Co-Investigator(Kenkyū-buntansha) |
高木 弘隆 国立感染症研究所, バイオセーフティ管理室, 研究員 (00332362)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | カリシウイルス / ノロウイルス / サポウイルス / リバースジェネティクス / 胆汁酸 / 感受性細胞 / ブタサポウイルス / ネコカリシウイルス / マウスノロウイルス |
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| Outline of Final Research Achievements |
We tested various cells and culture conditions to grow murine norovirus, human noroviruses, porcine sapovirus, and human sapoviruses to test the possibility of the virus propagation. During the study, an additional porcine sapovirus susceptible cell line was identified. Furthermore, several cells were susceptible for human norovirus or sapoviruses, because of detectable increase of viral RNA levels. Most of theses viruses and cells were required bile acid for virus propagation.Plasmid-based reverse genetics constructs were also applied for porcine sapovirus and feline calicivirus as an new molcular tool for virus production.
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