• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study on the responsible gene for the diminazene-resistant Babesia gibsoni using the DNA microarray.

Research Project

Project/Area Number 15K07739
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Veterinary medical science
Research InstitutionIwate University

Principal Investigator

Yamasaki Masahiro  岩手大学, 農学部, 教授 (40322846)

Co-Investigator(Kenkyū-buntansha) 今井 正樹  東京大学, 医科学研究所, 准教授 (30333363)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsバベシア症 / Babesia gibsoni / ジミナゼン製剤 / 薬剤耐性 / DNAマイクロアレイ / 次世代シークエンサー / ゲノム解析 / ジミナゼン / クローン化 / 次世代シークエンス / ジミナゼン・アセチュレート / RNAseq
Outline of Final Research Achievements

In this study, responsible genes for the diminazene-resistance of Babesia gibsoni, a blood protozoan in dogs, was analyzed. At first, we tried to use the DNA microarray for this investigation. However, there are less reference genes of B. gibsoni for this analysis. Therefore, we tried to use the next generation sequencer, which would not require the reference genes for the analysis. As a result, we obtained over 1,300 sequences, and huge numbers of partial sequence of genes of B. gibsoni.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (10 results)

All 2018 2017 2016 2015

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (6 results)

  • [Journal Article] Familial Congenital Methemoglobinemia in Pomeranian Dogs Caused by a Missense Variant in the NADH-Cytochrome B5 Reductase Gene2018

    • Author(s)
      Shino H.、Otsuka-Yamasaki Y.、Sato T.、Ooi K.、Inanami O.、Sato R.、Yamasaki M.
    • Journal Title

      Journal of Veterinary Internal Medicine

      Volume: 32 Issue: 1 Pages: 165-171

    • DOI

      10.1111/jvim.15031

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intracellular diminazene aceturate content and adenosine incorporation in diminazene aceturate-resistant Babesia gibsoni isolate in vitro2017

    • Author(s)
      Yamasaki Masahiro、Watanabe Nao、Idaka Natsuki、Yamamori Tohru、Otsuguro Ken-ichi、Uchida Naohiro、Iguchi Aiko、Ohta Hiroshi、Takiguchi Mitsuyoshi
    • Journal Title

      Experimental Parasitology

      Volume: 183 Pages: 92-98

    • DOI

      10.1016/j.exppara.2017.10.016

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning, phylogenetic analysis and heat shock response of <i>Babesia gibsoni</i> heat shock protein 902016

    • Author(s)
      Masahiro Yamasaki, Yoshihiro Tsuboi, Yusuke Taniyama, Naohiro Uchida, Reeko Sato, Kensuke Nakamura, Hiroshi Ohta, Mitsuyoshi Takiguchi
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 78 Issue: 8 Pages: 1355-1360

    • DOI

      10.1292/jvms.16-0027

    • NAID

      130005261509

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Molecular cloning, phylogenetic analysis and heat shock response of Babesia gibsoni heat shock protein 90.2016

    • Author(s)
      Yamasaki M, Tsuboi Y, Taniyama Y, Uchida N, Sato R, Nakamura K, Ohta H, Takiguchi M.
    • Journal Title

      The Journal of Veterinary Medical Science

      Volume: in press

    • NAID

      130005261509

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] イヌ、ヒツジおよびウシにおけるBabesia odocoilei様原虫のHSP70遺伝子の検出2017

    • Author(s)
      伊藤みのり、額田優花、井口愛子、内田直宏、小林沙織、佐藤れえ子、山崎真大
    • Organizer
      第86回日本寄生虫学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Babesia gibsoni感染犬における膵炎併発に関する回顧的研究2017

    • Author(s)
      益田美佐、井口愛子、内田直宏、佐藤れえ子、山崎真大
    • Organizer
      第38回動物臨床医学会年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 岩手県で発生したBabesia odocoilei様原虫を原因とする犬バベシア症の3例2017

    • Author(s)
      山崎真大、伊藤みのり、宍戸智、井口愛子、内田直宏、小林沙織、佐藤れえ子
    • Organizer
      第38回動物臨床医学会年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ジミナゼン耐性Babesia gibsoni株の細胞内ジミナゼン濃度の測定2016

    • Author(s)
      山﨑真大、渡邊夏央、井高菜月、大田寛、滝口満喜
    • Organizer
      第62回日本寄生虫学会・日本衛生動物学会北日本支部合同大会
    • Place of Presentation
      北里大学獣医学部(青森県十和田市)
    • Related Report
      2016 Research-status Report
  • [Presentation] LK型イヌ赤血球を宿主として用いたジミナゼン耐性Babesia gibsoniの作成2015

    • Author(s)
      登米美雪、堤弘夏、内田直宏、佐藤れえ子、山崎真大
    • Organizer
      第158回日本獣医学会学術集会
    • Place of Presentation
      北里大学獣医学部(青森県十和田市)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Research-status Report
  • [Presentation] Babesia gibsoniに対するHeat Shock Protein 90阻害剤の効果2015

    • Author(s)
      堤弘夏、登米美雪、内田直宏、佐藤れえ子、山崎真大
    • Organizer
      第158回日本獣医学会学術集会
    • Place of Presentation
      北里大学獣医学部(青森県十和田市)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi