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Study on vaccine using food allergen-producing lactic acid bacterium in food allergy

Research Project

Project/Area Number 15K07756
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Veterinary medical science
Research InstitutionAzabu University

Principal Investigator

Sakaguchi Masahiro  麻布大学, 獣医学部, 教授 (20170590)

Co-Investigator(Kenkyū-buntansha) 五十君 静信  国立医薬品食品衛生研究所, 食品衛生管理部, 客員研究員 (70212743)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords食物アレルギー / オボアルブミン / 乳酸菌 / イヌ / マウス / 卵 / アレルゲン
Outline of Final Research Achievements

Ovalbumin (OVA)-producing lactic acid bacteria (LB) were orally administrated to mice, followed by an intraperitoneal injection of OVA and alum. There were no differences in the levels of OVA-specific IgE between the group that received OVA-producing LB and the control groups, suggesting that the OVA-producing ability of these LB was low. Next, a mixture of OVA and LB was administered orally to mice. We found that the administration of more than 10 μg of OVA suppresses the levels of OVA-specific IgE. Mixtures of OVA and LB may therefore be used in immunotherapy for egg allergies. We then measured the levels of IgE specific to egg allergens in 82 dogs with food allergies. Approximately 10% of these dogs produced IgE specific to crude white egg. Furthermore, 75% of dogs with IgE specific to crude white egg also produced OVA-specific IgE. This suggests that a mixture of egg allergens and LB could be used in immunotherapy for food allergy in dogs.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (39 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (18 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 18 results,  Open Access: 18 results,  Acknowledgement Compliant: 3 results) Presentation (19 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Book (1 results)

  • [Int'l Joint Research] ウィスコンシン 大学(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Virus purification by CsCl density gradient using general centrifugation.2018

    • Author(s)
      Nasukawa T, Uchiyama J, Taharaguchi S, Ota S, Ujihara T, Matsuzaki S, Murakami H, Mizukami K, Sakaguchi M.
    • Journal Title

      Archives of Virology

      Volume: 162 Issue: 11 Pages: 3523-3528

    • DOI

      10.1007/s00705-017-3513-z

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Time dependent distinct roles of Toll-like receptor 4 in a house-dust-mite-induced asthma mouse model2018

    • Author(s)
      Ishii T, Niikura Y, Kurata K, Muroi M, Tanamoto K, Nagase T, Sakaguchi M, Yamashita N
    • Journal Title

      Scandinavian Journal of Immunology

      Volume: 87 Issue: 3 Pages: 1-7

    • DOI

      10.1111/sji.12641

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan.2018

    • Author(s)
      Ujihara T, Uchiyama J, Nasukawa T, Ando H, Murakami H, Ohara N, Ogawa M, Yamazaki T, Daibata M, Sakaguchi M, Matsuzaki S.
    • Journal Title

      Archives of Virology

      Volume: in press Issue: 7 Pages: 1-5

    • DOI

      10.1007/s00705-018-3788-8

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Piperacillin and ceftazidime produce the strongest synergistic phage-antibiotic effect in Pseudomonas aeruginosa.2018

    • Author(s)
      Uchiyama J, Shigehisa R, Nasukawa T, Mizukami K, Takemura-Uchiyama I, Ujihara T, Murakami H, Imanishi I, Nishifuji K, Sakaguchi M, Matsuzaki S.
    • Journal Title

      Archives of Virology

      Volume: in press Issue: 7 Pages: 1-8

    • DOI

      10.1007/s00705-018-3811-0

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of the PLA2G6 c.1579G>A missense mutation in Papillon dog neuroaxonal dystrophy using whole exome sequencing analysis.2017

    • Author(s)
      Tsuboi M, Watanabe M, Nibe K, Yoshimi N, Kato A, Sakaguchi M, Yamato O, Tanaka M, Kuwamura M, Kushida K, Ishikura T, Harada T, Chambers JK, Sugano S, Uchida K, Nakayama H.
    • Journal Title

      PLoS One

      Volume: 12 Issue: 1 Pages: 1-17

    • DOI

      10.1371/journal.pone.0169002

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Bovine leukemia virus G4 enhances virus production.2017

    • Author(s)
      Murakami H, Asano S, Uchiyama J, Sato R, Sakaguchi M, Tsukamoto K.
    • Journal Title

      Virus Research

      Volume: 238 Pages: 213-217

    • DOI

      10.1016/j.virusres.2017.07.005

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Adsorption of Staphylococcus viruses S13´ and S24-1 on Staphylococcus aureus strains with different glycosidic linkage patterns of wall teichoic acids.2017

    • Author(s)
      Uchiyama, J., Taniguchi, M., Kurokawa, K., Takemura-Uchiyama, I., Ujihara, T., Shimakura, H., Sakaguchi, Y., Murakami, H., Sakaguchi, M., Matsuzaki, S.
    • Journal Title

      J. Gen. Virol.

      Volume: 98(8) Issue: 8 Pages: 2171-2180

    • DOI

      10.1099/jgv.0.000865

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of Pseudomonas aeruginosa phage KPP21 belonging to family Podoviridae genus N4-like viruses isolated in Japan2016

    • Author(s)
      Shigehisa, R., Uchiyama, J., Kato S, Takemura-Uchiyama, I, Yamaguchi, K., Miyata, R., Ujihara, T., Sakaguchi, Y., Okamoto, N., Shimakura, H., Daibata, M., Sakaguchi, M., and Matsuzaki, S.
    • Journal Title

      Microbiology and Immunology

      Volume: 60 Issue: 1 Pages: 64-67

    • DOI

      10.1111/1348-0421.12347

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cross-reactivity between major IgE core epitopes on Cry j 2 allergen of Japanese cedar pollen and relevant sequences on Cha o 2 allergen of Japanese cypress pollen2016

    • Author(s)
      Miyaji, K., Okamoto, N., Saito, S., Yasueda, H., Takase, Y., Shimakura, H., Saito, S., and Sakaguchi, M.
    • Journal Title

      Allergology International

      Volume: 65 Issue: 3 Pages: 286-292

    • DOI

      10.1016/j.alit.2016.01.003

    • NAID

      130005165887

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analyses of Short-Term Antagonistic Evolution of Pseudomonas aeruginosa Strain PAO1 and Phage KPP22 (Myoviridae Family, PB1-Like Virus Genus).2016

    • Author(s)
      Uchiyama J, Suzuki M, Nishifuji K, Kato S, Miyata R, Nasukawa T, Yamaguchi K, Takemura-Uchiyama I, Ujihara T, Shimakura H, Murakami H, Okamoto N, Sakaguchi Y, Shibayama K, Sakaguchi M, Matsuzaki S.
    • Journal Title

      Appl Environ Microbiol

      Volume: 82 Issue: 15 Pages: 4482-4491

    • DOI

      10.1128/aem.00090-16

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Screening of KHP30-like prophages among Japanese Helicobacter pylori strains, and genetic analysis of a defective KHP30-like prophage sequence integrated in the genome of the H. pylori strain NY40.2016

    • Author(s)
      Uchiyama J, Takemura-Uchiyama I, Kato S, Takeuchi H, Sakaguchi Y, Ujihara T, Daibata M, Shimakura H, Okamoto N, Sakaguchi M, Matsuzaki S.
    • Journal Title

      FEMS Microbiol Lett.

      Volume: 363 Issue: 16 Pages: 1-9

    • DOI

      10.1093/femsle/fnw157

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] IgE reactivity to hen’s egg white allergens in dogs with cutaneous adverse food reactions.2016

    • Author(s)
      Shimakura, H., Saito, T., Uchiyama, J., Miyaji, K., Fujimura, M., Masuda, K., Okamoto, N., DeBoer, D.J., Sakaguchi, M.
    • Journal Title

      Veterinary Immunology and Immunopathology

      Volume: 177 Pages: 52-57

    • DOI

      10.1016/j.vetimm.2016.06.003

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Inefficient viral replication of bovine leukemia virus induced by spontaneous deletion mutation on G4 gene.2016

    • Author(s)
      Murakami, H., Uchiyama, J., Nikaido, S., Sato, R., Sakaguchi, M., Tsukamoto, K.
    • Journal Title

      Journal of General Virology

      Volume: 97 Issue: 10 Pages: 2753-2762

    • DOI

      10.1099/jgv.0.000583

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nationwide distribution of bovine influenza D virus infection in Japan.2016

    • Author(s)
      Horimoto T, Hiono T, Mekata H, Odagiri T, Lei Z, Kobayashi T, Norimine J, Inoshima Y, Hikono H, Murakami K, Sato R, Murakami H, Sakaguchi M, Ishii K, Ando T, Otomaru K, Ozawa M, Sakoda Y, Murakami S.
    • Journal Title

      PloSOne

      Volume: 11 (9) Issue: 9 Pages: 1-7

    • DOI

      10.1371/journal.pone.0163828

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cross-reactivity between major IgE core epitopes on Cry j 2 allergen of Japanese cedar pollen and relevant sequences on Cha o 2 allergen of Japanese cypress pollen2016

    • Author(s)
      Miyaji, K., Okamoto, N., Saito, S., Yasueda, H., Takase, Y., Shimakura, H., Saito, S., and Sakaguchi, M.
    • Journal Title

      Allergology International

      Volume: in press

    • NAID

      130005165887

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Analysis of KHP30-like prophage in the genome of Helicobacter 4 pylori strain NY40.2016

    • Author(s)
      Uchiyama, J , Takemura-Uchiyama, I., Kato, S., Takeuchi, H., Sakaguchi, Y., Ujihara, T., Daibata, M., Shimakura, H., Okamoto, N., Sakaguchi, M., Matsuzaki, S.
    • Journal Title

      FEMS Microbiology Letters

      Volume: in press

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Japanese Society of Allergology task force report on standardization of house dust mite allergen vaccines - Secondary publication2015

    • Author(s)
      Takai T, Okamoto Y, Okubo K, Nagata M, Sakaguchi M, Fukutomi Y, Saito A, Yasueda H, Masuyama K
    • Journal Title

      Allergology International

      Volume: 64 Issue: 2 Pages: 181-186

    • DOI

      10.1016/j.alit.2015.01.005

    • NAID

      130005290283

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cats as a potential source of emerging influenza virus infections2015

    • Author(s)
      Horimoto. T., Gen, F., Murakami, S., Iwatsuki-Horimoto, K., Kato, K., Hisasue, M., Sakaguchi, M., Nidom, C.A., and Kawaoka, Y
    • Journal Title

      Virologica Sinica

      Volume: 30 Issue: 3 Pages: 221-223

    • DOI

      10.1007/s12250-015-3580-9

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 遺伝的変化がもたらす牛白血病ウイルスの伝播力への影響2018

    • Author(s)
      村上裕信、内山淳平、鈴木千裕、二階堂紗恵、渋谷夏帆、佐藤礼一郎、前田洋佑、富岡美千子、竹嶋伸之輔、加藤肇、阪口雅弘、泉對博、間陽子、塚本健司
    • Organizer
      H29年度日本獣医師会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] イヌにおけるワクチン接種後のアレルギー副反応に関する疫学調査2018

    • Author(s)
      伊藤 雅人,水上 圭二郎,内山 淳平, 阪口 雅弘
    • Organizer
      第14回日本獣医内科アカデミー
    • Related Report
      2017 Annual Research Report
  • [Presentation] 花粉飛散時期における室内飼育犬の被毛への花粉付着と除去の検討2018

    • Author(s)
      白井 秀治, 中村 恒彰, 磯田 由美, 相場 美咲, 黒崎 勉, 阪口 雅弘
    • Organizer
      第14回日本獣医内科アカデミー
    • Related Report
      2017 Annual Research Report
  • [Presentation] Study of adsorption of AHJD-like phages infecting Staphylococcus aureus2017

    • Author(s)
      Jumpei Uchiyama, Kenshi Kurokawa, Iyo Takemura-Uchiyama, Takako Ujihara, Yoshihiko Sakaguchi, Masahiro Sakaguchi, Shigenobu Matsuzaki
    • Organizer
      第90回日本細菌学会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] 犬アトピー性皮膚炎症例におけるEnterococcus faecalis FK-23投与と臨床症状の変化にする研究 多施設実施二重盲検無作為プラセボ比較試験2017

    • Author(s)
      大隅尊史、阪口雅弘、嶋田貴志、辻本元
    • Organizer
      第20回獣医皮膚科学学会
    • Place of Presentation
      大宮
    • Year and Date
      2017-03-11
    • Related Report
      2016 Research-status Report
  • [Presentation] 実験動物アレルギーにおける診断と治療2017

    • Author(s)
      阪口雅弘
    • Organizer
      第94回実験動物コンファレンス
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 普遍形質導入による黄色ブドウ球菌の進化可能性検討2017

    • Author(s)
      内山淳平、内山伊代、中森洋佑、水上圭二郎、阪口雅弘、松崎茂展
    • Organizer
      MRSAフォーラム2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Analysis of adsorption of phages, S13and and S24-1, belonging to the family Podoviridae genus P68virus, using Staphylococcus aureus strains with different glycosidic linkage patterns of wall teichoic acids2017

    • Author(s)
      Uchiyama J, Kurokawa K, Takemura-Uchiyama I, Ujihara T, Sakaguchi Y, Sakaguchi M, Matsuzaki S
    • Organizer
      International Union of Microbiological Societies 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Virus purification by CsCl density gradient using general centrifugation2017

    • Author(s)
      Nasukawa T, Uciyama J, Taharaguchi S, Ota S, Ujihara T, Matsuzaki S, Murakami H, Mizukami K, Sakaguchi M
    • Organizer
      16th Awaji International Forum on Infection and Immunity
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ワンヘルスにおけるヒトと動物のアレルギー疾患2017

    • Author(s)
      阪口雅弘
    • Organizer
      第1回日本ワンヘルスサイエンス学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 花粉飛散時期における室内飼育犬の被毛への花粉付着の検討2017

    • Author(s)
      白井秀治、中村恒彰、磯田由美、相場美咲、阪口雅弘
    • Organizer
      第160回日本獣医学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 日本における伴侶動物の遺伝子関連疾患研究のための犬の遺伝子(DNA)バンクについて2017

    • Author(s)
      阪口雅弘
    • Organizer
      第160回日本獣医学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 犬のアトピー性皮膚炎の遺伝的解析2017

    • Author(s)
      村上裕信、佐々木慎二、阪口雅弘
    • Organizer
      第160回日本獣医学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] H7亜型鳥インフルエンザを迅速かつ網羅的に検出するLAMP法の開発2017

    • Author(s)
      野口きらら 、高橋ゆきの 、古山雄平 、久松伸 、阪口雅弘 、村上裕信 、塚本健司
    • Organizer
      第160回日本獣医学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The Mutant Allele frequency of the canine TUBB1:c.5G>A Associated with Asymptomatic Macrothrombocytopenia in Norfolk and Cairn Terriers in Japan2017

    • Author(s)
      Kinjo S, Takahashi M, Yamato O, Sakaguchi M, Mizukami K, Tsujimoto H, Endo Y
    • Organizer
      Asia Meeting of Animal Medicine Specialities 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DogFilerを用いた犬種推定法の検討2016

    • Author(s)
      旭 愛、岡本憲明、関口和正、阪口雅弘、武内ゆかり、松田秀明
    • Organizer
      第22回日本法科学技術学会
    • Place of Presentation
      東京
    • Year and Date
      2016-11-10
    • Related Report
      2016 Research-status Report
  • [Presentation] Serological survey reveals the wide distribution of bovine influenza D virus infection in Japan2016

    • Author(s)
      Taisuke Horimoto, Tomoha Odagiri, Zhihao Lei, Tomoya Kobayashi, Maiko Endo, Akiko Uema, Hirohisa Mekata, Junzo Norimine, Kenji Murakami, Hironobu Murakami, Masahiro Sakaguchi, Kazunori Ishii, Yasuo Inoshima, Makoto Ozawa, Takahiro Hiono, Yoshihiro Sakoda, Benjamin Hause, Shin Murakami
    • Organizer
      第64回日本ウイルス学会
    • Place of Presentation
      札幌
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report
  • [Presentation] ダニアレルゲンワクチン標準化に関する日本アレルギー学会タスクフォース報告2016

    • Author(s)
      高井敏朗、岡本美孝、大久保)公裕、永田真、阪口雅弘、福冨友馬、齋藤明美、安枝、浩、増山敬祐
    • Organizer
      第65回日本アレルギー学会
    • Place of Presentation
      東京
    • Year and Date
      2016-06-17
    • Related Report
      2016 Research-status Report
  • [Presentation] 食物有害反応を示す犬における卵白抗原に対するIgE反応性の検討2015

    • Author(s)
      島倉秀勝、齋藤拓、藤村正人 、岡本憲明、内山淳平、阪口雅弘
    • Organizer
      第158回日本獣医学会
    • Place of Presentation
      十和田
    • Year and Date
      2015-09-07
    • Related Report
      2015 Research-status Report
  • [Book] 有害微生物の制御と管理2016

    • Author(s)
      阪口雅弘
    • Total Pages
      664
    • Publisher
      テクノシステム
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2022-02-16  

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