Regulation of three dimensional formation of renal tubule by Stat1

• Project/Area Number: 15K07763
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Arai Katsuhiko 東京農工大学, 農学部, 教授 (60175940)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 腎尿細管 / Stat1 / MDCK / カドヘリン16 / Stat / 腎尿細管細胞 / 腎特異的カドヘリン / CDH16 / HGF

Outline of Final Research Achievements

Effect of wild-type or dominant negative form of Stat1 on tubule formation of canine renal epithelial cell-derived MDCK cells was examined. Wild-type Stat1 upregulated expression of kidney-specific cadherin 16 and induced formation of rigid and narrow tubule, while dominant negative Stat1-transfectant formed dilated and thin-walled tubule.

Research Products

• [Journal Article] Pattern Abnormalities in the Green Sea Turtle from the Ogasawara Archipelago, Japan. (2017)
  • Author(s): Kobayashi, S, Morimoto, Y, Kondo, S, Sato, T, Suganuma, H, Arai, K, Watanabe, G.
  • Journal Title: Zoological Sci. Volume: 34 Issue: 4 Pages: 281-286
  • DOI: 10.2108/zs160159
  • Peer Reviewed / Open Access
• [Journal Article] Degradation of staphylococcal enterotoxin A by a Pseudomonas aeruginosa isolate from raw milk. (2017)
  • Author(s): Fujikawa, H, Nagaoka, K, Arai, K.
  • Journal Title: Biosci Biotechnol Biochem. Volume: 81 Issue: 7 Pages: 1436-1443
  • DOI: 10.1080/09168451.2017.1314755
  • Peer Reviewed / Open Access
• [Journal Article] The effects of nest incubation temperature on embryos and hatchlings of the loggerhead sea turtle: Implications of sex difference for survival rates during early life stages. (2017)
  • Author(s): Kobayashi S, Wada S, Fujimoto R, Kumazawa Y, Arai K, Watanabe G, Saito T.
  • Journal Title: J Exp Mar Bio Ecol. Volume: 486 Pages: 274-281
  • DOI: 10.1016/j.jembe.2016.10.020
  • Peer Reviewed / Open Access
• [Journal Article] Degradation of staphylococcal enterotoxin A by a Pseudomonas aeruginosa isolate from raw milk (2017)
  • Author(s): Fujikawa H, Nagaoka K, Arai K.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 印刷中
  • Peer Reviewed / Open Access
• [Journal Article] Sex differences and the heritability of scute pattern abnormalities in the green sea turtle from the Ogasawara archipelago, Japan (2017)
  • Author(s): Kobayashi S, Morimoto Y, Kondo S, Sato T, Suganuma H, Arai K, Watanabe G.
  • Journal Title: Zoological Sci. Volume: 印刷中
  • NAID: 40021289609
  • Peer Reviewed / Open Access
• [Journal Article] The effects of nest incubation temperature on embryos and hatchlings of the loggerhead sea turtle: Implications of sex difference for survival rates during early life stages. (2017)
  • Author(s): Kobayashi S, Wada S, Fujimoto R, Kumazawa Y, Arai K, Watanabe G, Saito T.
  • Journal Title: J. Exp. Mar. Bio. Ecol. Volume: 486
  • Peer Reviewed / Open Access
• [Journal Article] Fibronectin modified expression of Sonic hedgehog in ATRA-mediated neuronal differentiation (2015)
  • Author(s): Arahara K, Matsumoto T, Morimatsu F, Arai K.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 11 Pages: 1503-1506
  • DOI: 10.1292/jvms.15-0122
  • NAID: 130005112106
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Population doubling level-dependent change of secreted glycosaminoglycan in equine bone marrow-derived mesenchymal stem cells (2015)
  • Author(s): 2.Sasao T, Fukuda Y, Yoshida S, Miyabara S, Kasashima Y, Kuwano A, Arai K
  • Journal Title: Journal of Equine Science Volume: 26 Issue: 3 Pages: 73-80
  • DOI: 10.1294/jes.26.73
  • NAID: 130005101257
  • ISSN: 1340-3516, 1347-7501
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] ウマの浅指屈筋健損傷に対する幹細胞移植治療の基礎研究と臨床応用 (2018)
  • Author(s): 笠嶋 快周,田村 周久,黒田 泰輔,福田 健太郎,新井 克彦
  • Organizer: 第17回日本再生医療学会総会
  • Invited
• [Presentation] Development of a vaccine against human coronavirus using gold nanoparticles (2017)
  • Author(s): H SekimukaiHanako, N Iwata-Yoshikawa, A Fukuma, S Fukushi, H Tani, M Kataoka,K Arai, K Niikura, H Hasegawa, N Nagata
  • Organizer: ConBio 2017
• [Presentation] Cell adhesion inhibitory protein in the nematocyte of Aurelia sp. (2017)
  • Author(s): A Shindo, H Yamada, M Kusuhata, S Hattroi, K Arai
  • Organizer: ConBio 2017
• [Presentation] Molecular cloning of mesoglein, jellyfish-specific ECM protein (2017)
  • Author(s): K. Arai, K. Tsukimori
  • Organizer: ConBio 2017
• [Presentation] Expression and characterization of human coronavirus spike proteins associated with severe pneumonia (2017)
  • Author(s): H Sekimukai, N Iwata-Yoshikawa, A Fukuma, S Fukushi, H Tani, K Arai, K Niikura , C Takeuchi , K Nanbara , M Takeda , H Hasegawa, N Nagata
  • Organizer: XIVth International Nidovirus Symposium
  • Int'l Joint Research
• [Presentation] 東京都小笠原諸島におけるアオウミガメ(Chelonia mydas)の鱗板配列奇形を誘発する原因 (2016)
  • Author(s): 小林翔平、森元由佳里、佐藤隆行、近藤理美、菅沼弘行、新井克彦、渡辺元
  • Organizer: 第27回日本ウミガメ会議 室戸大会
  • Place of Presentation: 高知
  • Year and Date: 2016-12-10
• [Presentation] Stem cell therapy for tendon injury of racehorses in Japan: Present conditions and future prospects (2016)
  • Author(s): Y Kasashima, N Tamura, A Tomita, K Fukuda, T Kuroda, T Tabata and K Arai
  • Organizer: Havemeyer conference on regenerative medicine II
  • Place of Presentation: Florida, USA
  • Year and Date: 2016-11-05
  • Int'l Joint Research / Invited
• [Presentation] 動物間葉系幹細胞の品質管理に用いるマーカー候補分子に対するモノクローナル抗体スクリーニング法の開発① (2016)
  • Author(s): 中島奈緒、新井克彦、荻野智絵、佐藤耕太、大石弘司、能田 健
  • Organizer: 第159回日本獣医学会
  • Place of Presentation: 日本大学・藤沢キャンパス
  • Year and Date: 2016-09-06
• [Presentation] 動物間葉系幹細胞の品質管理に用いるマーカー候補分子に対するモノクローナル抗体スクリーニング法の開発② (2016)
  • Author(s): 荻野智絵、新井克彦、中島奈緖、佐藤耕太、大石弘司、能田健
  • Organizer: 第159回日本獣医学会
  • Place of Presentation: 日本大学・藤沢キャンパス
  • Year and Date: 2016-09-06
• [Presentation] ウマ骨髄由来間葉系幹細胞における酸性ムコ多糖分泌の 集団倍加レベル依存性の変化 (2015)
  • Author(s): 笹尾貴文, 福田有希, 吉田沙也子, 宮原志穂璃, 笠嶋快周, 桑野睦敏, 新井克彦
  • Organizer: 日本ウマ科学会 第28回学術集会
  • Place of Presentation: 東京大学農学部
  • Year and Date: 2015-11-30
• [Presentation] イヌ腎尿細管由来細胞株MDCKのStat1依存性の管腔形成並びに腎特異的カドヘリン16発現制御 (2015)
  • Author(s): 大竹詩織，吉田沙也子，新井克彦
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学獣医学部
  • Year and Date: 2015-09-08
• [Presentation] ミズクラゲと共通抗原性を持つ哺乳類分子の特性 (2015)
  • Author(s): 石原豊，小西良，前澤汐見，小林正行，多賀祐喜，町田登，服部俊治，新井克彦
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学獣医学部
  • Year and Date: 2015-09-08
• [Presentation] マウス胚性腫瘍細胞株のフィブロネクチンによるソニックヘッジホッグ遺伝子発現の制御 (2015)
  • Author(s): 高橋智，荒原一彦，新井克彦
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学獣医学部
  • Year and Date: 2015-09-08
• [Book] 改訂獣医生化学 (2016)
  • Author(s): 新井 克彦
  • Total Pages: 255
  • Publisher: 朝倉書店
• [Book] 改訂 獣医生化学 (2016)
  • Author(s): 新井克彦
  • Total Pages: 256
  • Publisher: 朝倉書店
• [Remarks] 教職員活動データベース
  • URL: https://kenkyu-db.tuat.ac.jp/scripts/update/kkmain.htm
• [Remarks] 国立大学法人東京農工大学 研究者プロフィール
  • URL: http://kenkyu-web.tuat.ac.jp/Profiles/2/0000175/profile.html