Analysis of modification mechanism of reproductive regulation focusing on OGR1 family receptor

• Project/Area Number: 15K07772
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Meiji University
• Principal Investigator: Tomura Hideaki 明治大学, 農学部, 専任教授 (70217553)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: OGR1 / ゴナドトロフ / プロトン / 生殖調節 / OGR1ファミリー受容体 / 性腺

Outline of Final Research Achievements

Reproductive regulation is controlled along the hypothalamic-pituitary-gonadal axis pathway. Here we show that OGR1 is expressed in rat pituitary gonadotropic cells and a mouse gonadotropic cell line. We also revealed that OGR1 is involved in activation of gene promoter accompanying pH decrease in the gonadotropic cell line. Furthermore, it was suggested that OGR1 may be involved in hormone secretion response due to pH decrease. These results suggest that OGR1 may be involved in reproductive regulation.

Research Products

• [Journal Article] Metals differentially activate ovarian cancer G protein-coupled receptor 1 in various species. (2018)
  • Author(s): 6.Mochimaru Y, Negishi J, Murakami S, Musha S, Sato K, Okajima F, Tomura H.
  • Journal Title: Zoolog Sci. Volume: 35 Issue: 2 Pages: 109-114
  • DOI: 10.2108/zs170145
  • NAID: 40021514985
  • Peer Reviewed
• [Journal Article] Manganese and cobalt activate zebrafish ovarian cancer G-protein-coupled receptor 1 but not GPR4 (2017)
  • Author(s): J. Negishi, Y. Omori, M. Shindo, H. Takanashi, S. Musha, S. Nagayama, J. Hirayama, H. Nishina, T. Nakakura, C. Mogi, K. Sato, F. Okajima, Y. Mochimaru, H. Tomura
  • Journal Title: J. Recept. Signal Transduct. Res. Volume: 37 Issue: 4 Pages: 401
  • DOI: 10.1080/10799893.2017.1298130
  • Peer Reviewed / Open Access
• [Journal Article] Easy detection of hormone secretion from LβT2 cells by using Gaussia luciferase (2017)
  • Author(s): Satou K, Mochimaru Y, Nakakura T, Kusada T, Negishi J, Musha S, Yoshimura N, Kato Y, Tomura H
  • Journal Title: Journal of Reproduction and Development Volume: 63 Issue: 2 Pages: 199-204
  • DOI: 10.1262/jrd.2016-174
  • NAID: 130007314627
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed
• [Journal Article] Two zebrafish G2A homologs activate multiple intracellular signaling pathways in acidic environment (2016)
  • Author(s): Y. Ichijo, Y. Mochimaru, M. Azuma, K. Satou, J. Negishi, T. Nakakura, N. Oshima, C. Mogi, K. Sato, K. Matsuda, F. Okajima, H. Tomura
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 469 Issue: 1 Pages: 81
  • DOI: 10.1016/j.bbrc.2015.11.075
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of imidazopyridine compounds as negative allosteric modulators of proton-sensing GPR4 in extracellular acidification-induced responses (2015)
  • Author(s): Ayaka Tobo, Masayuki Tobo, Takashi Nakakura, Masashi Ebara, Hideaki Tomura, Chihiro Mogi, Dong-Soon Im, Naoya Murata, Atsushi Kuwabara, Saki Ito, Hayato Fukuda, Mitsuhiro Arisawa, Satoshi Shuto, Michio Nakaya, Hitoshi Kurose, Koichi Sato, Fumikazu Okajima
  • Journal Title: PLoS One. Volume: 10 Issue: 6 Pages: e0129334-e0129334
  • DOI: 10.1371/journal.pone.0129334
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] G-protein-coupled receptor 4受容体のリガンド及び機能 (2015)
  • Author(s): 大嶋菜月、戸村秀明
  • Journal Title: 明治大学農学部研究報告 Volume: 65 Pages: 1-7
  • NAID: 120005676965
  • Peer Reviewed
• [Journal Article] 創薬のターゲットとしてのovarian cancer G-protein-coupled receptor 1 (2015)
  • Author(s): 持丸雄太、戸村秀明
  • Journal Title: 明治大学農学部研究報告 Volume: 65 Pages: 9-16
  • NAID: 120005676966
  • Peer Reviewed
• [Presentation] Effect of metal ions on the activation of zebrafish ovarian cancer G-protein-coupled receptor 1 and GPR4 (2017)
  • Author(s): Negishi J, Musha S, Nagayama S, Tomura H.
  • Organizer: Fourth World Congress of Reproductive Biology
  • Int'l Joint Research
• [Presentation] Metal ions may modulate protoninduced OGR1 activation (2017)
  • Author(s): Mochimaru Y, Murakami S, Yoshimura N, Tomura H.
  • Organizer: Fourth World Congress of Reproductive Biology
  • Int'l Joint Research
• [Presentation] OGR1の金属応答性は生物種間で異なる. (2017)
  • Author(s): 持丸雄太、戸村秀明
  • Organizer: 第32回日本下垂体研究会学術集会
• [Presentation] ACTH産生細胞株におけるGPHRの機能解析 (2017)
  • Author(s): 村上奨、持丸雄太、戸村秀明
  • Organizer: 第32回日本下垂体研究会学術集会
• [Presentation] ゼブラフィッシュOGR1, GPR4の金属による応答解析 (2017)
  • Author(s): 武者詩織、根岸潤、永山純礼、持丸雄太、戸村秀明
  • Organizer: 第32回日本下垂体研究会学術集会
• [Presentation] 金属イオンによるOGR1, GPR4を介した応答解析 (2017)
  • Author(s): 武者詩織、根岸潤、永山純礼、持丸雄太、戸村秀明
  • Organizer: Conbio2017
• [Presentation] 金属イオンによるOGR1活性化の差を生む部位の探索. (2017)
  • Author(s): 村上奨、持丸雄太、東野瑚子、吉村名央、戸村秀明
  • Organizer: Conbio2017
• [Presentation] 生物種による各金属イオンによるOGR1活性化様式の多様性. (2016)
  • Author(s): 鳥海拓也, 持丸雄太, 金子涼, 吉村名央, 東野瑚子, 戸村秀明.
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-11-30
  • Int'l Joint Research
• [Presentation] ゼブラフィッシュOGR1は金属イオンにより活性化される (2016)
  • Author(s): 高梨颯, 根岸潤, 大森由花, 武者詩織, 永山純礼, 戸村秀明
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-11-30
  • Int'l Joint Research
• [Presentation] 生物種間における金属イオンによるOGR1活性化の比較. (2016)
  • Author(s): 持丸雄太, 根岸潤, 大森由花, 高梨颯, 武者詩織, 戸村秀明
  • Organizer: 第109回日本繁殖生物学会大会
  • Place of Presentation: 麻布大学
  • Year and Date: 2016-09-11
  • Int'l Joint Research
• [Presentation] Effect of metal ions on the activation of zebrafish ovarian cancer G-protein-coupled receptor 1 (2016)
  • Author(s): Negishi J, Omori Y, Nagayama S, Tomura H.
  • Organizer: International Symposium on Pituitary Gland and Related Systems
  • Place of Presentation: ホノルル・ハワイ
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] Proton activates OGR1, GPR4 and G2A homologs of zebrafish (2016)
  • Author(s): Mochimaru Y, Azuma M, Negishi J, Tomura H.
  • Organizer: International Symposium on Pituitary Gland and Related Systems
  • Place of Presentation: ホノルル・ハワイ
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] Proton activates OGR1, GPR4 and G2A homologs of zebrafish.1 (2016)
  • Author(s): Mochimaru Y, Azuma M, Negishi J, Tomura H.
  • Organizer: International Society for Stem Cell Research
  • Place of Presentation: サンフランシスコ・カリフォルニア
  • Year and Date: 2016-06-22
  • Int'l Joint Research
• [Presentation] Metals activate ovarian cancer G-protein-coupled receptor 1 of zebrafish (2015)
  • Author(s): Jun Negishi, Yuka Omori, Hayate Takanashi, Tomoyuki Kusada, Hideaki Tomura
  • Organizer: CompBiol 2015広島大会
  • Place of Presentation: JMSアステールプラザ（広島）
  • Year and Date: 2015-12-11
• [Presentation] Analysis of LbT2 cell responses through ovarian cancer G-protein coupled receptor 1 (2015)
  • Author(s): Yuta Mochimaru, Kazuhiro Satou, Mami Shindo, Yukio Kato, Hideaki Tomura
  • Organizer: CompBiol 2015広島大会
  • Place of Presentation: JMSアステールプラザ（広島）
  • Year and Date: 2015-12-11
• [Presentation] ガウシアルシフェラーゼを利用した高感度ホルモン分泌アッセイ系の構築の試み (2015)
  • Author(s): 佐藤一裕，根岸潤，中倉敬，草田智之，加藤幸雄, 戸村秀明
  • Organizer: 第108回日本繁殖生物学会
  • Place of Presentation: 宮崎大学
  • Year and Date: 2015-09-17
• [Presentation] プロトン刺激による性腺刺激ホルモン産生細胞株の応答解析 (2015)
  • Author(s): 持丸雄太, 新堂真実, 西田真実, 金子諒、加藤幸雄, 戸村秀明
  • Organizer: 第108回日本繁殖生物学会
  • Place of Presentation: 宮崎大学
  • Year and Date: 2015-09-17
• [Presentation] ガウシアルシフェラーゼのLβT2細胞におけるホルモン分泌アッセイ系の構築への利用 (2015)
  • Author(s): 佐藤一裕，根岸潤，中倉敬，草田智之，大森由花, 加藤幸雄, 戸村秀明
  • Organizer: 第30回日本下垂体研究会
  • Place of Presentation: 宇奈月国際会館セレネ（富山）
  • Year and Date: 2015-08-05
• [Presentation] プロトン刺激によるマウス下垂体細胞株LβT2の応答解析 (2015)
  • Author(s): 持丸雄太, 新堂真実, 西田真実, 金子諒, 加藤幸雄, 戸村秀明
  • Organizer: 第30回日本下垂体研究会
  • Place of Presentation: 宇奈月国際会館セレネ（富山）
  • Year and Date: 2015-08-05