Investigation of the mechanism of the Ca2+ dependent exocrine pancreatic secretion by optogenetic control

• Project/Area Number: 15K07782
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Juntendo University
• Principal Investigator: Nakamura Kyoko 順天堂大学, 医学部, 非常勤助教 (90578858)
• Co-Investigator(Kenkyū-buntansha): 濱田 耕造 国立研究開発法人理化学研究所, 脳科学総合研究センター, 研究員 (00311358)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: カルシウムシグナリング / ムスカリン作動性アセチルコリン受容体 / 光刺激 / ムスカリニックアセチルコリン受容体 / 膵外分泌機能

Outline of Final Research Achievements

Intracellular Ca2+ plays important roles as triggers for multiple biological phenomena. The aim of the present study was to clarify the role of intracellular Ca2+ for the release of digestive enzymes from pancreatic acinar cells. We found mAChR subtypes-dependent characteristic patterns of Ca2+ dynamics in the cell induced by an agonist (ACh). However, the relationship between the Ca2+ dynamics and the enzyme-release remain unknown. In the present study, we tried to get some clues to rule out the relationship between the enzyme release and the intracellular Ca2+ dynamics by an optogenetic method.