| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Protein glycosylation is involved in several biological functions via complex formation with target proteins and glycoproteins. To comprehensively understand biological roles of the glycan-glycoprotein complex, it is important to identify interaction sites in complexes as well as to reveal structural changes related to complex formation. However, many issues still remain in general higher-order structural analytical methods, including X-ray crystal structural analysis and NMR. These methods require complicated operations, such as crystallization and preparation of high-concentration sample solutions. We have analyzed protein-protein interactions under physiological conditions via hydrogen deuterium exchange reaction/liquid chromatography/mass spectrometry (HDX/MS). In this study, we evaluated the effectiveness of the HDX/MS using model glycan and glycoproteins. We demonstrated that HDX/MS is useful as an easy and rapid analytical method for glycan-glycoprotein interaction.
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