Development of analytical method for glycoprotein-glycan interaction by hydrogen/deuterium exchange mass spectrometry
| Project/Area Number |
15K07916
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
Hashii Noritaka 国立医薬品食品衛生研究所, 生物薬品部, 室長 (20425672)
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| Co-Investigator(Kenkyū-buntansha) |
鈴木 琢雄 国立医薬品食品衛生研究所, 生物薬品部, 主任研究官 (10415466)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 水素重水素交換／質量分析 / 相互作用解析 / 質量分析 / 水素重水素交換 / 糖鎖 / 糖タンパク質 / HDX/MS |
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| Outline of Final Research Achievements |
Protein glycosylation is involved in several biological functions via complex formation with target proteins and glycoproteins. To comprehensively understand biological roles of the glycan-glycoprotein complex, it is important to identify interaction sites in complexes as well as to reveal structural changes related to complex formation. However, many issues still remain in general higher-order structural analytical methods, including X-ray crystal structural analysis and NMR. These methods require complicated operations, such as crystallization and preparation of high-concentration sample solutions. We have analyzed protein-protein interactions under physiological conditions via hydrogen deuterium exchange reaction/liquid chromatography/mass spectrometry (HDX/MS). In this study, we evaluated the effectiveness of the HDX/MS using model glycan and glycoproteins. We demonstrated that HDX/MS is useful as an easy and rapid analytical method for glycan-glycoprotein interaction.
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Report
(4 results)
Research Products
(4 results)
