Identification and characterization of the cell surface marker protein that can distinguish between hematopoietic and myocardial progenitor cells

• Project/Area Number: 15K07961
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: National Institutes of Biomedical Innovation, Health and Nutrition
• Principal Investigator: KAWABATA Kenji 国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所 幹細胞制御プロジェクト, プロジェクトリーダー (50356234)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 分化 / iPS細胞 / 中胚葉 / 血液 / 心筋 / 心筋細胞 / 血液細胞 / マーカー

Outline of Final Research Achievements

Coxsackievirus and adenovirus receptor (CAR) was identified as adenovirus entry receptor. We found that there were CAR-positive and -negative cells in mesodermal (Flk-1 positive) cells derived from human ES and iPS cells. Flk-1-positive and CAR-positive cells could differentiate into myocardial progenitor cells. On the other hand, Flk-1-positive and CAR-negative cells could differentiate into hematopoietic and endothelial progenitor cells. These results suggest that CAR is an appropriate cell surface marker that can distinguish between hematopoietic and myocardial progenitor cells.

Research Products

• [Journal Article] Expression of coxsackievirus and adenovirus receptor separates hematopoietic and cardiac progenitor cells in fetal liver kinase 1-expressing mesoderm. (2015)
  • Author(s): Tashiro K, Hirata N, Okada A, Yamaguchi T, Takayama K, Mizuguchi H, Kawabata K.
  • Journal Title: Stem Cells Transl. Med. Volume: 4 Issue: 5 Pages: 424-436
  • DOI: 10.5966/sctm.2014-0173
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 中胚葉分化誘導法の開発と標準化 (2016)
  • Author(s): 川端健二
  • Organizer: 日本組織培養学会第89回大会
  • Place of Presentation: 大阪
  • Year and Date: 2016-05-25
  • Invited
• [Presentation] ヒトiPS細胞からマスト細胞への分化誘導と特性解析 (2016)
  • Author(s): 山口朋子、西島美妙江、川端健二
  • Organizer: 第15回日本再生医療学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2016-03-17
• [Presentation] Characterization of connective tissue-type mast cells derived from human iPS cells (2015)
  • Author(s): Tomoko Yamaguchi, Kenji Kawabata
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2015-11-18
• [Presentation] Differentiation of human iPS cell-derived hematopoietic progenitor cells into mast cells. (2015)
  • Author(s): 2.Tomoko Yamaguchi, Reiko Hirabayashi, Katsuhisa Tashiro, Atsumasa Okada, Hiroyuki Mizuguchi, Kenji Kawabata
  • Organizer: International Society for Stem Cell Research 13th Annual Meeting
  • Place of Presentation: ストックホルム
  • Year and Date: 2015-06-24
  • Int'l Joint Research