Role of thermo-sensitive TRPV1 and TRPM8 channels in visceral hypersensitivity in inflammatory bowel disease model animals and ulcerative colitis patients
| Project/Area Number |
15K07968
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pharmacology in pharmacy
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| Research Institution | Josai International University |
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Principal Investigator |
Horie Syunji 城西国際大学, 薬学部, 教授 (50209285)
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| Research Collaborator |
HOSOYA Takuji
YAMAKAWA Takumi
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 潰瘍性大腸炎 / 温度感受性TRPチャネル / 知覚過敏 / 痛覚過敏 / 知覚神経 / 過敏性腸症候群 / TRPV1 / TRPM8 / 内蔵知覚過敏 / 一次求心性知覚神経 / 免疫組織化学 / 好中球 / 病態モデルマウス / 機能性ディスペプシア / 病態モデル / 遠位結腸 / 求心性一次知覚神経 |
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| Outline of Final Research Achievements |
We investigated the involvement of TRPM8 in hyperalgesia in normal and experimental colitis and irritable bowel syndrome (IBS) model animals.
TRPM8 immunoreactivity in the distal colon was much higher than in the transverse and proximal colon under physiological conditions. TRPM8 immunoreactivity markedly increased in the distal colon mucosa of colitis model mice compared with the control mice. The number of TRPM8 nerve fibers in mucosa of colitis model mice drastically increased compared with the control. Intracolonic administration of WS-12 induced behavioral visceral pain-like responses. The numbers of these responses in the colitis model were 3 times higher than in the control, and were decreased by pretreatment with the TRPM8 channel blocker AMTB. In IBS model, the number of TRPM8 nerve fibers in mucosa of butyrate-induced IBS model also increased compared with the control. Increased expression of TRPM8 may contribute to the visceral hyperalgesia of experimental colitis and IBS.
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Report
(4 results)
Research Products
(42 results)
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[Presentation] Allyl isothiocyanate, a pungent ingredient of wasabi, induces gastric low-grade inflammation in rats to lead to the impaired gastric motility; involvement of prostaglandin, capsaicin-sensitive sensory neurons, and nitric oxide2017
Author(s)
Kimihito Tashima, Shizuki Yamaura, Kazuki Hashimoto, Yasuhiro Mori, Tomoharu Shinoki, Yuki Ishihara, Syunji Horie
Organizer
Digestive Disease Week 2017
Related Report
Int'l Joint Research
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[Book] アルカロイドの科学2017
Author(s)
高山廣光編、堀江俊治分担執筆
Total Pages
560
Publisher
化学同人
ISBN
9784759814187
Related Report
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