Identification of innate immune ligands to induce the protection from MRSA infection

• Project/Area Number: 15K08056
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental and hygienic pharmacy
• Research Institution: Nagasaki International University
• Principal Investigator: Kurokawa Kenji 長崎国際大学, 薬学部, 准教授 (80304963)
• Research Collaborator: LEE Bok-Leul 釜山大学校, 薬学部, 教授 ; TAKAHASHI Kazue Massachusetts General Hospital, Assistant Professor
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 細菌宿主相互作用 / 樹状細胞 / 黄色ブドウ球菌 / 免疫賦活化 / 細胞壁タイコ酸

Outline of Final Research Achievements

Staphylococcus aureus infection induces IL-23 that is critical for its biological defense, however the bacterial ligand molecules for the IL-23-induction have been unknown. We established a bioassay system to evaluate the IL-23 inducing activity by using a cultured human dendritic cell line and found that there were at least two molecules for the induction; one was wall teichoic acids (WTA) and the other was unidentified. Biochemical analyses using cell wall fractions derived from WTA modification enzymes revealed that a complete modified structure of WTA was not necessary for the IL-23 secretion.

Research Products

• [Int'l Joint Research] Pusan National University(韓国)
• [Int'l Joint Research] Massachusetts General Hospital(米国)
• [Int'l Joint Research] Pusan National University(韓国)
• [Int'l Joint Research] Massachusetts General Hospital(米国)
• [Int'l Joint Research] Pusan National University(韓国)
• [Int'l Joint Research] Massachusetts General Hospital(米国)
• [Journal Article] Adsorption of Staphylococcus viruses S13´ and S24-1 on Staphylococcus aureus strains with different glycosidic linkage patterns of wall teichoic acids. (2017)
  • Author(s): Uchiyama, J., Taniguchi, M., Kurokawa, K., Takemura-Uchiyama, I., Ujihara, T., Shimakura, H., Sakaguchi, Y., Murakami, H., Sakaguchi, M., Matsuzaki, S.
  • Journal Title: J. Gen. Virol. Volume: 98(8) Issue: 8 Pages: 2171-2180
  • DOI: 10.1099/jgv.0.000865
  • Peer Reviewed / Open Access
• [Journal Article] The staphylococcal surface-glycopolymer wall teichoic acid (WTA) is crucial for complement activation and immunological defense against Staphylococcus aureus infection. (2016)
  • Author(s): Kurokawa K, Takhashi K, and Lee BL.
  • Journal Title: Immunobiology Volume: 221 Issue: 10 Pages: 1091-1101
  • DOI: 10.1016/j.imbio.2016.06.003
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Surface glycopolymers are crucial for in vitro anti-wall teichoic acid IgG-mediated complement activation and opsonophagocytosis of Staphylococcus aureus. (2015)
  • Author(s): Lee JH, Kim NH, Winstel V, Kurokawa K, Larsen J, An JH, Khan A, Seong MY, Lee MJ, Andersen PS, Peschel A, and Lee BL.
  • Journal Title: Infect Immun. Volume: 83 Issue: 11 Pages: 4247-4255
  • DOI: 10.1128/iai.00767-15
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ヒト血清による黄色ブドウ球菌の認識と貪食の誘導機構 (2015)
  • Author(s): 黒川健児
  • Journal Title: KAGAKU TO SEIBUTSU Volume: 53 Issue: 5 Pages: 319-325
  • DOI: 10.1271/kagakutoseibutsu.53.319
  • NAID: 130005146581
  • ISSN: 0453-073X, 1883-6852
  • Peer Reviewed / Open Access
• [Presentation] Analysis of adsorption of phages, S13' and S24-1, belonging to the family Podoviridae genus P68virus, using Staphylococcus aureus strains with different glycosidic linkage patterns of wall teichoic acids. (2017)
  • Author(s): Uchiyama J, Kurokawa K, Takemura-Uchiyama I, Ujihara T, Sakaguchi Y, Sakaguchi M, and Matsuzaki S.
  • Organizer: International Union of Microbiology Societies (IUMS) 2017 congresses
  • Int'l Joint Research
• [Presentation] Staphylococcus viruses S13' and S24-1 on Staphylococcus aureus strains with different glycosidic linkage patterns of wall teichoic acids. (2017)
  • Author(s): Uchiyama J, Taniguchi M, Kurokawa K, Takemura-Uchiyama I, Ujihara T, Shimakura H, Sakaguchi Y, Murakami H, Sakaguchi M, and Matsuzaki S.
  • Organizer: The 16th Awaji International Forum on Infection and Immunity
  • Int'l Joint Research
• [Presentation] Surface-glycopolymers are crucial for anti-WTA IgG-mediated complement activation and opsonophagocytosis of Staphylococcus aureus. (2015)
  • Author(s): Lee JH, Kim NH, Kurokawa K, and Lee BL.
  • Organizer: 第52回日本補体学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-08-21