| Project/Area Number |
15K08063
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | Kanagawa Institute of Industrial Sclence and Technology (2017)
Kanagawa Academy of Science and Technology (2015-2016) |
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Principal Investigator |
Hirooka Takashi 地方独立行政法人神奈川県立産業技術総合研究所, 食品機能性評価グループ, 研究員(任期有) (50397519)
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| Co-Investigator(Kenkyū-buntansha) |
大森 清美 神奈川県衛生研究所, 理化学部, 主任研究員 (20416069)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 発がん性予測試験 / 細胞形質転換試験 / Bhas42 細胞 / 肝代謝 / ヒト肝細胞株 / 発がんプロモーター / 代謝活性化 / CYP酵素 / Bhas42細胞 / 発がんイニシエーター / 発がんプロモーション / 発がんイニシエーション |
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| Outline of Final Research Achievements |
The Bhas42 cell transformation assay (Bhas42 CTA) has been internationally certified as an in vitro test method for predicting of chemical carcinogenicity. The purpose of this study is the development of the cell transformation assay using Bhas42 cells co-cultured with human hepatocytes for predicting the carcinogenicity of chemicals metabolized by hepatic metabolism system. We made the modified Bhas42 CTA medium, in which the CYP 3A4 activity of the human hepatocyte could be maintained at a detectable level for 10 days. Moreover, significantly increase in the formation of focus wasn’t observed in Bhas42 cells cultured with the modified CTA medium in the absence of test chemicals. On the other hand, we showed the expression of CYP1A1, 1A2 and 2B6 in Bhas42 cells by western blot analysis and CYP activity assay, and further demonstrated the contribution of CYP activities to focus formation of Bhas42 cells in initiation assay of 3-methylcholanthrene.
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