Development of a drug delivery system targeting activated neutrophils
| Project/Area Number |
15K08082
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | トランスポーター / リソソーム / 化学発光 / D-ルシフェリン / 細胞膜 / OAT1 / 好中球 |
|---|
| Outline of Final Research Achievements |
We demonstrated that the properties of granule membrane present in differentiated HL-60 cells were similar to those of lysosomes in most types of cells, and stimulus response with ionomycin resulted in exocytosis of granules to fuse with plasma membrane. In order to quantify the expression level of transporter in plasma membrane with high sensitivity real-time monitoring, we established the system to image bioluminescence derived from D-luciferin-luciferase reaction via OAT1 transport of D-luciferin. This system with the expressing of OAT1 in lysosomes could allow to quantify their fusion to plasma membrane, and provide the possibility of drug delivery via lysosomal transporters.
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Report
(4 results)
Research Products
(13 results)
