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Clarification of drug resistance of mucoid type Streptococcus pneumoniae

Research Project

Project/Area Number 15K08103
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Medical pharmacy
Research InstitutionSapporo Medical University

Principal Investigator

Miyamoto Atsushi  札幌医科大学, 医学部, 教授 (50166196)

Co-Investigator(Kenkyū-buntansha) 横田 伸一  札幌医科大学, 医学部, 教授 (10325863)
白石 宗  札幌医科大学, 医学部, 助教 (70725168)
北川 学  札幌医科大学, 医学部, 研究員 (70751270)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords肺炎球菌 / ムコイド / 抗菌ペプチド / 納豆由来ペプチド / MIC / 抗菌性物質 / バイオフィルム / MBEC / 薬剤抵抗性
Outline of Final Research Achievements

We obtained 51 mucoid strains from 1,061 Streptococcus pneumoniae clinical isolates collected in Hokkaido Prefecture, Japan. We evaluated biofilm formation, minimum biofilm eradication concentration of various antimicrobials, and growth rate. Significant differences between mucoid and non-mucoid strains were not observed. On the other hand, we found that Natto peptide, which is from a Japanese soy bean fermented food, showed specific bactericidal activity against S. pneumoniae, and Bacillus subtilis group of Bacillus genus. We clarified that its mechanism was failure of cell separation during cell division in S. pneumoniae. The Natto peptide showed similar antimicrobial activity against mucoid, non-mucoid, antimicrobial susceptible, and multidrug resistant strains of S. pneumoniae.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (10 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) Book (1 results)

  • [Journal Article] Novel antimicrobial activities of a peptide derived from a Japanese soybean fermented food, Natto, against Streptococcus pneumoniae and Bacillus subtilis group strains.2017

    • Author(s)
      Manabu Kitagawa, Tsukasa Shiraishi, Soh Yamamoto, Ryosuke Kutomi, Yasuo Ohkoshi, Toyotaka Sato, Hideki Wakui, Hideaki Itoh, Atsushi Miyamoto, Shin-ichi Yokota.
    • Journal Title

      AMB Express

      Volume: 7 Issue: 1 Pages: 127-127

    • DOI

      10.1186/s13568-017-0430-1

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Adaptive Cross-Resistance to Aminoglycoside Antibiotics in Pseudomonas aeruginosa Induced by Topical Dosage of Neomycin2017

    • Author(s)
      Shunji Uemura, Shin-ichi Yokota, Tsukasa Shiraishi, Manabu Kitagawa, Suguru Hirayama, Ryoko Kyan, Hirotoshi Mizuno, Keigo Sawamoto, Hiroyuki Inoue, Atsushi Miyamoto, Eichi Narimatsu
    • Journal Title

      Chemotherapy

      Volume: 62 Issue: 2 Pages: 121-127

    • DOI

      10.1159/000449368

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Plasma tenofovir trough concentrations are associated with renal dysfunction in Japanese patients with HIV infection: a retrospective cohort study2016

    • Author(s)
      Yusuke Kunimoto, Hiroshi Ikeda, Satoshi Fujii, Manabu Kitagawa, Kieko Yamazaki, Hiromasa Nakata, Norimasa Noda, Tadao Ishida, Atsushi Miyamoto
    • Journal Title

      Journal of Pharmaceutical Health Care and Sciences

      Volume: 2 Issue: 1 Pages: 22-22

    • DOI

      10.1186/s40780-016-0056-5

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Novel antimicrobial activities of a peptide derived from a Japanese soy-bean fermented food, Natto2017

    • Author(s)
      北川 学、白石 宗、山本 聡、久富 亮佑、佐藤 豊孝、小笠原 徳子、宮本 篤、横田 伸一
    • Organizer
      第90回日本細菌学会総会
    • Place of Presentation
      仙台国際センター 展示棟
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] 納豆由来ペプチドの特異な殺菌活性と作用機序2017

    • Author(s)
      白石宗、北川学、山本聡、久富亮佑、佐藤豊孝、涌井秀樹、伊藤英晃、宮本篤、横田伸一
    • Organizer
      第11回細菌学若手コロッセウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 納豆抽出物由来ペプチドの特異な殺菌作用2016

    • Author(s)
      白石 宗、横田 伸一
    • Organizer
      第22回 日本エンドトキシン・自然免疫研究会
    • Place of Presentation
      鹿児島大学 郡元キャンパス
    • Year and Date
      2016-12-02
    • Related Report
      2016 Research-status Report
  • [Presentation] 納豆抽出物由来ペプチドの殺菌作用の特異性2016

    • Author(s)
      北川 学、白石 宗、山本 聡、佐藤 豊孝、小笠原 徳子、宮本 篤、横田 伸一
    • Organizer
      第83回日本細菌学会北海道支部会
    • Place of Presentation
      北海道大学大学院歯学研究科・歯学部講堂
    • Related Report
      2016 Research-status Report
  • [Presentation] 抗菌薬処理による緑膿菌菌体から遊離するリポ多糖量の変化2015

    • Author(s)
      袴田浩、山本聡、佐藤豊孝、白石宗、小笠原徳子、北川学、宮本篤、横田伸一
    • Organizer
      日本細菌学会北海道支部学術総会
    • Place of Presentation
      北海道医療大学心理科学部講義室4-5
    • Year and Date
      2015-09-05
    • Related Report
      2015 Research-status Report
  • [Presentation] チゲサイクリン非感受性大腸菌はフルオロキノロン耐性株に多く認められる2015

    • Author(s)
      佐藤豊孝、鈴木裕樹、大越康雄、山本聡、小笠原徳子、白石宗、田村豊、横田伸一
    • Organizer
      日本細菌学会北海道支部学術総会
    • Place of Presentation
      北海道医療大学心理科学部講義室4-5
    • Year and Date
      2015-09-05
    • Related Report
      2015 Research-status Report
  • [Book] エンドトキシン・自然免疫研究20自然免疫における化学生物学の貢献2017

    • Author(s)
      白石宗、北川学、山本聡、久富亮佑、佐藤豊孝、涌井秀樹、伊藤英晃、宮本篤、横田伸一
    • Total Pages
      82
    • Publisher
      医学図書出版
    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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