Mechanisms underlying development of ependymal cells that constitute brain-CSF barrier

• Project/Area Number: 15K08127
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Tohoku University
• Principal Investigator: Hiraoka Kotaro 東北大学, サイクロトロン・ラジオアイソトープセンター, 助教 (70586049)
• Co-Investigator(Renkei-kenkyūsha): OSUMI Noriko 東北大学, 医学(系)研究科(研究院), 教授 (00220343)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 脳 / 脳脊髄液系 / 上衣細胞 / 発達・分化 / BMPシグナリング / 脳脊髄液 / 免疫組織化学 / 細胞培養 / 分化・成熟 / 免疫組織染色

Outline of Final Research Achievements

In the brain, there are spaces called "the ventricles". The ventricles contain fluid called "the cerebrospinal fluid (CSF)". The ventricles are covered by layers of the ependymal cells. The cells regulate transfer of various molecules between the brain and CSF. The ependymal cells protrude hairlike structures called "the cilia" to the ventricles. Motility of the cilia affects dynamics of the CSF. In development of the brain, it is not fully clarified which factors induce development of the ependymal cells from stem cells to mature cells. In this study, we analyzed association between bone morphogenetic protein, a protein used in the body for signaling, and development of the ependymal cells. We also analyzed association between the CSF and development of the ependymal cells.

Research Products

• [Journal Article] Effects of levocetirizine and diphenhydramine on regional glucose metabolic changes and hemodynamic responses in the human prefrontal cortex during cognitive tasks. (2018)
  • Author(s): Kikuchi A, Nasir FBM, Inami A, Mohsen A, Watanuki S, Miyake M, Takeda K, Koike D, Ito T, Sasakawa J, Matsuda R, Hiraoka K, Maurer M, Yanai1 K, Watabe H, Tashiro M.
  • Journal Title: Human Psychopharmacology. Volume: 33 Issue: 2
  • DOI: 10.1002/hup.2655
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Correlations of (18)F-THK5351 PET with Postmortem Burden of Tau and Astrogliosis in Alzheimer Disease. (2018)
  • Author(s): Harada R, Furukawa K, et al.
  • Journal Title: J Nucl Med Volume: 59 Issue: 4 Pages: 671-674
  • DOI: 10.2967/jnumed.117.197426
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The role of Pax6 in brain development and its impact on pathogenesis of autism spectrum disorder (2018)
  • Author(s): Kikkawa Takako、Casingal Cristine R.、Chun Seung Hee、Shinohara Hiroshi、Hiraoka Kotaro、Osumi Noriko
  • Journal Title: Brain Research Volume: - Pages: 30111-2
  • DOI: 10.1016/j.brainres.2018.02.041
  • Peer Reviewed
• [Journal Article] Glucose Metabolic Changes in the Brain and Muscles of Patients with Nonspecific Neck Pain Treated by Spinal Manipulation Therapy: A [18F]FDG PET Study (2017)
  • Author(s): Inami A, Ogura T, Watanuki S, Masud MM, Shibuya K, Miyake M, Matsuda R, Hiraoka K, Itoh M, Fuhr AW, Yanai K, Tashiro M
  • Journal Title: Evid Based Complement Alternat Med. Volume: 2017 Issue: 1 Pages: 1-9
  • DOI: 10.1155/2017/4345703
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] In vivo visualization of tau deposits in corticobasal syndrome by 18F-THK5351 PET (2016)
  • Author(s): Kikuchi A, Okamura N, Hasegawa T, Harada R, Watanuki S, Funaki Y, Hiraoka K, Baba T, Sugeno N, Oshima R, Yoshida S, Kobayashi J, Ezura M, Kobayashi M, Tano O, Mugikura S, Iwata R, Ishiki A, Furukawa K, Arai H, Furumoto S, Tashiro M, Yanai K, Kudo Y, Takeda A, Aoki M
  • Journal Title: Neurology Volume: 87 Pages: 2309-2316
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Brain Metabolic Changes of Cervical Dystonia with Spinocerebellar Ataxia Type 1 after Botulinum Toxin Therapy (2016)
  • Author(s): Kikuchi A, Takeda A, Sugeno N, Miura E, Kato K, Hasegawa T, Baba T, Konno M, Oshima R, Watanuki S, Hiraoka K, Tashiro M, Aoki M
  • Journal Title: Internal Medicine Volume: 55 Issue: 14 Pages: 1919-1922
  • DOI: 10.2169/internalmedicine.55.5843
  • NAID: 130005164997
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Dynamic PET measures of tau accumulation in cognitively normal older adults and Alzheimer's disease patients measured using [18F]THK-5351. (2016)
  • Author(s): Lockhart SN, Baker SL, Okamura N, Furukawa K, Ishiki A, Furumoto S, Tashiro M, Yanai K, Arai H, Kudo Y, Harada R, Tomita N, Hiraoka K, Watanuki S, Jagust WJ.
  • Journal Title: PLoS One. Volume: 11 Issue: 6 Pages: e0158460-e0158460
  • DOI: 10.1371/journal.pone.0158460
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Regional volume decreases in the brain of Pax6 heterozygous mutant rats: MRI deformation-based morphometry (2016)
  • Author(s): Hiraoka, K., Sumiyoshi, A., Nonaka, H., Kikkawa, T., Kawashima, R. and Osumi, N.
  • Journal Title: PLoS One Volume: 11 Issue: 6 Pages: e0158153-e0158153
  • DOI: 10.1371/journal.pone.0158153
  • Peer Reviewed / Open Access
• [Journal Article] A simulated car-driving study on the effects of acute administration of levocetirizine, fexofenadine, and diphenhydramine in healthy Japanese volunteers. (2016)
  • Author(s): Inami A, Matsuda R, Grobosch T, Komamura H, Takeda K, Yamada Y, Miyake M, Hiraoka K, Maurer M, Yanai K, Tashiro M.
  • Journal Title: Hum Psychopharmacol. Volume: Epub ahead of print Issue: 3 Pages: 167-77
  • DOI: 10.1002/hup.2524
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 18F-THK5351: A Novel PET Radiotracer for Imaging Neurofibrillary Pathology in Alzheimer Disease (2016)
  • Author(s): Harada R, Okamura N, Furumoto S, Furukawa K, Ishiki A, Tomita N, Tago T, Hiraoka K, Watanuki S, Shidahara M, Miyake M, Ishikawa Y, Matsuda R, Inami A, Yoshikawa T, Funaki Y, Iwata R, Tashiro M, Yanai K, Arai H, Kudo Y
  • Journal Title: J Nucl Med Volume: 57 Issue: 2 Pages: 208-214
  • DOI: 10.2967/jnumed.115.164848
  • Peer Reviewed
• [Journal Article] 抗ヒスタミン薬の効果 薬理学から～PETを用いたヒスタミンH1受容体占拠率評価と運転パフォーマンス試験～ (2016)
  • Author(s): 稲見暁恵、平岡宏太良、谷内一彦、田代学
  • Journal Title: アレルギー・免疫 Volume: 23 Pages: 36-43
• [Journal Article] Histamine H receptor occupancy by the new-generation antipsychotics olanzapine and quetiapine: a positron emission tomography study in healthy volunteers (2015)
  • Author(s): Sato, Hirotoshi Ito, Chihiro Hiraoka, Kotaro Tashiro, Manabu Shibuya, Katsuhiko Funaki, Yoshihito Yoshikawa, Takeo Iwata, Ren Matsuoka, Hiroo Yanai, Kazuhiko
  • Journal Title: Psychopharmacology (Berl) Volume: 232 Issue: 19 Pages: 3497-3505
  • DOI: 10.1007/s00213-015-4002-2
  • Peer Reviewed
• [Journal Article] Amyloid deposits and response to shunt surgery in idiopathic normal-pressure hydrocephalus (2015)
  • Author(s): Hiraoka, Kotaro Narita, Wataru Kikuchi, Hirokazu Baba, Toru Kanno, Shigenori Iizuka, Osamu Tashiro, Manabu Furumoto, Shozo Okamura, Nobuyuki Furukawa, Katsutoshi Arai, Hiroyuki Iwata, Ren Mori, Etsuro Yanai, Kazuhiko
  • Journal Title: J Neurol Sci Volume: 356 Issue: 1-2 Pages: 124-128
  • DOI: 10.1016/j.jns.2015.06.029
  • Peer Reviewed
• [Journal Article] Longitudinal Assessment of Tau Pathology in Patients with Alzheimer's Disease Using [18F]THK-5117 Positron Emission Tomography (2015)
  • Author(s): Ishiki, Aiko Okamura, Nobuyuki Furukawa, Katsutoshi Furumoto, Shozo Harada, Ryuichi Tomita, Naoki Hiraoka, Kotaro Watanuki, Shoichi Ishikawa, Yoichi Tago, Tetsuro Funaki, Yoshihito Iwata, Ren Tashiro, Manabu Yanai, Kazuhiko Kudo, Yukitsuka Arai, Hiroyuki
  • Journal Title: PLoS One Volume: 10 Issue: 10 Pages: 1-9
  • DOI: 10.1371/journal.pone.0140311
  • Peer Reviewed / Open Access
• [Presentation] Regional volume decreases in the brain of Pax6 heterozygous mutant rats: MRI deformation-based morphometry (2016)
  • Author(s): Kotaro Hiraoka Akira Sumiyoshi Hiroi Nonaka Takako Kikkawa Ryuta Kawashima Noriko Osumi
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Presentation] シンポジウム1 (iNPHの疫学）「iNPHは希少疾患か？Common diseaseか？－本邦の疫学研究から。」 講演1. Community-based studies 1)宮城県の研究 (2016)
  • Author(s): 平岡宏太良
  • Organizer: 第17回日本正常圧水頭症学会
  • Place of Presentation: 山形市
  • Year and Date: 2016-03-19
  • Invited
• [Presentation] Pax6変異ヘテロ接合ラットにおける脳体積の減少―小動物用MRIを用いた脳形態解析 (2015)
  • Author(s): 平岡宏太良
  • Organizer: 第１１回成体脳のニューロン新生懇談会
  • Place of Presentation: 名古屋市
  • Year and Date: 2015-11-14
• [Presentation] Decreased volume in the brain of Pax6 heterozygous mutant rats: A morphometric MRI study (2015)
  • Author(s): Kotaro Hiraoka, Akira Sumiyoshi , Hiroi Nonaka , Ryuta Kawashima , Noriko Osumi
  • Organizer: Symposium on Development and Disease
  • Place of Presentation: 仙台市
  • Year and Date: 2015-08-24
  • Int'l Joint Research