| Project/Area Number |
15K08136
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Ehime University |
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Principal Investigator |
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| Research Collaborator |
Kitazawa Sohei 愛媛大学, 大学院医学系研究科, 教授
Kitazawa Riko 愛媛大学, 医学部附属病院, 特任教授
Takaoka Yuki 愛媛大学, 大学院医学系研究科, 技術補佐員
Shiraishi chie 愛媛大学, 大学院医学系研究科, 技術補佐員
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 成長板軟骨 / ヘッジホッグ / 遺伝子改変マウス / genetic lineage tracing / 組織系譜解析 / 骨形成 / 成長板 / 骨格形成 / Genetic Lineage Tracing / 成長軟骨板 / ヘッジホッグシグナル |
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| Outline of Final Research Achievements |
The endochondral ossification process at the growth plate is governed by paracrine signals secreted from terminally differentiated chondrocytes (hypertrophic chondrocytes), and hedgehog signaling is one of the best known regulatory signaling pathways in this process. We carried out genetic lineage tracing with the use of Gli1CreERT2 mice line in order to follow the fate of hedgehog signal-responsive cells during endochondral bone formation. Gli1CreERT2 genetically labeled cells are detected in hypertrophic chondrocytes and osteo-progenitors at the chondro-osseous junction (COJ); these progeny then commit to the osteogenic lineage in periosteum, trabecular and cortical bone along the developing longitudinal axis. Our study show, for the first time, evidence of the developmental contribution of endochondral progenitors under the influence of epiphyseal chondrocyte-derived secretory signals in longitudinally growing bone.
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