| Project/Area Number |
15K08144
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Tsukuba International University |
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Principal Investigator |
Kazuhiko Sawada つくば国際大学, 医療保健学部, 教授(移行) (10284324)
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| Co-Investigator(Kenkyū-buntansha) |
廣瀬 美和 (堀内美和) 茨城キリスト教大学, 看護学部, 准教授 (90381714)
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| Co-Investigator(Renkei-kenkyūsha) |
AOKI Ichio 量子科学技術研究開発機構, 量子MRI研究グループ, チームリーダー (10319519)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | cerebral cortex / subventricular zone / neural stem cells / basal radial glia / ferret / sulcus / gyrus / MRI / 大脳皮質 / 脳室下帯 / 神経幹細胞 / フェレット / 脳溝 / 脳回 / バルプロ酸 / 免疫組織化学 |
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| Outline of Final Research Achievements |
Ferrets were received ip injection of valpronic acid (VPA) on postnatal days (PDs) 5 to 7. Brains were removed on PD 20 and T1W-MRI was acquired ex vivo. By MRI-based morphometry, both volumes and surface areas of the cerebral cortex did not differ on PD 20 between VPA-treated and control ferrets. The degree of gyrification was significantly lesser in VPA-treated ferrets. In particular, sulcal infolding was smaller in the cingulate and frontal cortical regions of VPA-treated ferrets. In those regions, a significantly greater cortical thickness was obtained in VPA-treated ferrets. By immunofluorescence analysis, late-generated neurons undergoing multiple rounds of cell divisions were densely distributed in the upper cortical layers of the prefrontal, cingulate and parietooccipital regions of 20-day-old ferrets. The present results suggest that the folding of particular cortical regions is reduced by thickening the cortex by exposure to VPA during late neurogenesis in ferrets.
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