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Infrastructure development for experimental animalization of ancient fish Polypterus as an ancestor model of tetrapods

Research Project

Project/Area Number 15K08145
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionJikei University School of Medicine

Principal Investigator

Okabe Masataka  東京慈恵会医科大学, 医学部, 教授 (10300716)

Co-Investigator(Kenkyū-buntansha) 矢野 十織  東京慈恵会医科大学, 医学部, 助教 (10648091)
Research Collaborator TATSUMI Norifumi  
NAGASAWA Tatsuki  
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsポリプテルス / 肺 / 外鰓 / 鰭 / 進化 / 発生 / 形態 / 形態形成 / 遺伝子組換え
Outline of Final Research Achievements

Polypterus senegalus (bichir) is a member of the Actinopterygii; the larva has external gill and the adult uses lung for respiration. To establish the bichir bioresource in Japan, we have developed breeding systems and transgenic/gene-editing methods, for a stable supply of bichir embryos and larvae. We have investigated mechanisms of development of respiratory organs (external gill and lung) and the morphology of locomotor organs (appendages).

Academic Significance and Societal Importance of the Research Achievements

本課題で実施したゲノム科学的・分子発生学的アプローチからの進化発生学的研究の成果は、新聞・メディア・学会や論文公表を通じて多数発信することができ、ポリプテルスが実験動物として身近なものになるよう努めた。文化的背景から、進化学研究は欧米よりも日本で推進しやすい環境にあり、また子供たちを含む社会一般からも注目される科学分野である。本邦医学部の基礎医学・解剖学分野からこうした進化学研究の情報発信ができたことは、国民の科学リテラシーを高める貢献ができたと考える。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (21 results)

All 2019 2018 2017 2016 2015

All Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] Development of cranial muscles in the actinopterygian fish Senegal bichir, Polypterus senegalus Cuvier, 1829.2017

    • Author(s)
      Noda M, Miyake T, Okabe M.
    • Journal Title

      J Morphol

      Volume: 278 Issue: 4 Pages: 450-463

    • DOI

      10.1002/jmor.20636

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular developmental mechanism in polypterid fish provides insight into the origin of vertebrate lungs.2016

    • Author(s)
      Norifumi Tatsumi, Ritsuko Kobayashi, Tohru Yano, Masatsugu Noda, Koji Fujimura, Norihiro Okada & Masataka Okabe
    • Journal Title

      Scientific Reports

      Volume: July Issue: 1 Pages: 1-10

    • DOI

      10.1038/srep30580

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Evolutionary changes in the developmental origin of hatching gland cells in basal ray-finned fishes.2016

    • Author(s)
      Nagasawa T, Kawaguchi M, Yano T, Sano K, Okabe M, Yasumasu S.
    • Journal Title

      Zoolog Sci.

      Volume: 33(3) Issue: 3 Pages: 272-81

    • DOI

      10.2108/zs150183

    • NAID

      210000187339

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evolution of the fish heart by sub/neofunctionalization of an elastin gene.2016

    • Author(s)
      Moriyama Y., Ito F., Takeda H., Yano T., Okabe M., Kuraku S., Keeley FW. and Koshiba-Takeuchi K.
    • Journal Title

      Nature Communications.

      Volume: 7 Issue: 1 Pages: 10397-10397

    • DOI

      10.1038/ncomms10397

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evolutionary changes in the developmental origin of hatching gland cells in basal ray-finned fishes.2016

    • Author(s)
      Nagasawa T., Kawaguchi M., Yano T., Sano K., Okabe M., and Yasumasu S.
    • Journal Title

      Zoological Science

      Volume: 未定

    • NAID

      210000187339

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evidence for an amphibian sixth digit.2015

    • Author(s)
      Hayashi S., Kobayashi T., Yano T., Kamiyama N., Egawa S., Seki R., Takizawa K., Okabe M., Yokoyama H. and Tamura K.
    • Journal Title

      Zoological Letters.

      Volume: 1 Issue: 1 Pages: 17-17

    • DOI

      10.1186/s40851-015-0019-y

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 条鰭類の鰭条骨関節の組織学形態と分子特性2019

    • Author(s)
      矢野十織, 岡部正隆
    • Organizer
      日本動物学会第71回支部大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] MALDI-TOFを用いたポリプテルス側線感丘形成予定表皮の網羅的分子解析2018

    • Author(s)
      井上龍太郎、重谷安代、岩本武夫、岡部正隆
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 原始魚類ポリプテルスの実験動物化を目指した基盤整備2017

    • Author(s)
      矢野十織、長澤竜樹、西野弘嵩、岡部正隆
    • Organizer
      第122回日本解剖学会総会・全国学術集会
    • Place of Presentation
      長崎
    • Year and Date
      2017-03-27
    • Related Report
      2016 Research-status Report
  • [Presentation] ポリプテルスの体躯側線感丘は発生を通じて表層に見られる2017

    • Author(s)
      重谷安代、矢野十織、岡部正隆
    • Organizer
      第122回日本解剖学会総会・全国学術集会
    • Place of Presentation
      長崎
    • Year and Date
      2017-03-27
    • Related Report
      2016 Research-status Report
  • [Presentation] 肺が先、鰾が後 ポリプテルスでわかった肺、鰾の進化2017

    • Author(s)
      辰巳徳史、小林律子、西野弘嵩、長澤竜樹、矢野十織、野田真継、藤村衡至、岡田典弘、岡部正隆
    • Organizer
      第122回日本解剖学会総会・全国学術集会
    • Place of Presentation
      長崎
    • Year and Date
      2017-03-27
    • Related Report
      2016 Research-status Report
  • [Presentation] Transition of basal lamina during lateral line migration in Polypterus2017

    • Author(s)
      Shigetani Y, Inoue R, Okabe M.
    • Organizer
      2017年度生命科学系学会合同年次大会(ConBio2017)
    • Related Report
      2017 Research-status Report
  • [Presentation] 脊椎動物の進化発生学研究における「古代魚」ポリプテルス2016

    • Author(s)
      岡部正隆
    • Organizer
      第39 回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] The conserved enhancer of the hatching enzyme gene in ray-finned fish2016

    • Author(s)
      Nagasawa T, Yano T, Kawaguchi M, Yasumasu S, Okabe M
    • Organizer
      Joint meeting of the 22nd International Congress of Zoology
    • Place of Presentation
      沖縄
    • Year and Date
      2016-11-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Morphogenesis of lateral line neuromast in the trunk of Polypterus2016

    • Author(s)
      Shigetani Y, Yano T, Okabe M
    • Organizer
      The 17th International Symposium on Olfaction and Taste
    • Place of Presentation
      横浜
    • Year and Date
      2016-06-05
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 脊椎動物の形態進化研究における古代魚ポリプテルスへの期待2016

    • Author(s)
      岡部正隆
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      ビッグパレットふくしま(郡山)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 脊椎動物の上陸と呼吸器の進化2016

    • Author(s)
      岡部正隆
    • Organizer
      第39回日本嚥下医学会総会
    • Place of Presentation
      大阪国際交流センター(大阪)
    • Year and Date
      2016-02-12
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Morphogenesis of the lateral line neuromast in the primitive fish Polypterus.2015

    • Author(s)
      Yasuyo Shigetani, Tohru Yano, and Masataka Okabe
    • Organizer
      第38回日本分子生物学会年会第88回日本生化学学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド(神戸)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 孵化腺細胞は2種類の下位条鰭類の間で異なる胚葉から分化する2015

    • Author(s)
      長澤竜樹、矢野十織、川口眞理、岡部正隆、安増茂樹
    • Organizer
      第86回日本動物学会
    • Place of Presentation
      朱鷺メッセ(新潟)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Research-status Report
  • [Presentation] 脊椎動物孵化腺細胞の発生進化学的解析2015

    • Author(s)
      長澤竜樹、川口眞理、矢野十織、岡部正隆、安増茂樹
    • Organizer
      日本進化学会第17回大会
    • Place of Presentation
      中央大学後楽園キャンパス(東京)
    • Year and Date
      2015-08-20
    • Related Report
      2015 Research-status Report
  • [Book] 動物学の百科事典2018

    • Author(s)
      矢野十織, 阿部玄武, 岡部正隆, 田村宏冶
    • Total Pages
      2
    • Publisher
      丸善出版
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2020-03-30  

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