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Physiological role of KCa1.1 channel in ER membrane

Research Project

Project/Area Number 15K08169
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General physiology
Research InstitutionTohoku University

Principal Investigator

Murata Yoshimichi  東北大学, 医学系研究科, 講師 (60455780)

Research Collaborator Maruyama Yoshio  
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsイオンチャネル / 小胞体
Outline of Final Research Achievements

Ion channels working at plasma membrane are assembled in endoplasmic reticulum, then they are transported to their destination via Golgi apparatus. But it is unclear that whether they are conferred any function(s) and physiological role(s) in the intracellular membrane.
We observed large conductance Ca2+-activated K+ (MaxiK) currents in nuclear envelopes (peri-nuclear ER membrane) of pancreatic acinar cells and HEK293 cells using the patch-clamp technique. Consequently we tried to elucidate role(s) of this channel in ER membrane. We evaluated alteration in free Ca2+ content in the ER expressing MaxiK channel and indicated that MaxiK-expressing HEK293 cells have larger Ca2+ content in ER than wild type HEK293 cells.

Academic Significance and Societal Importance of the Research Achievements

近年、神経疾患や糖尿病、急性膵炎などの疾患に小胞体の機能異常が関与することが明らかになるなど、小胞体の生理的、病理的役割が注目されている。しかし、小胞体内のイオン環境に着目した研究はほとんどない。
本研究では、K+チャネルにより(イオン透過を介さずに)小胞体Ca2+イオン濃度が変化するという現象の発見、ER内pH変化の観察手法の確立などに至った。これらの成果は、ER内のイオン環境研究の端緒となり、小胞体機能のさらなる理解、生理的・病理的役割の解明に繋がるものである。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2019 2018 2017 2016

All Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Presentation] ATP dependent H+ transport in endoplasmic reticulum membrane2019

    • Author(s)
      村田喜理。丸山芳夫
    • Organizer
      FAOPS2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] K+チャネルの小胞体膜における機能の解明2018

    • Author(s)
      村田喜理、丸山芳夫
    • Organizer
      第3回イオンチャネル研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ATP dependent proton transport in endoplasmic reticulum2018

    • Author(s)
      村田喜理、丸山芳夫
    • Organizer
      第49回生理研国際シンポジウム Ion channels: looking back, seeing ahead
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 小胞体に存在するK+チャネルによる小胞体Ca2+ストアの増加2017

    • Author(s)
      村田喜理
    • Organizer
      第13回TRPチャネル研究会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Evaluation of alteration in the endoplasmic reticulum function with MaxiK channels2017

    • Author(s)
      村田喜理
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      アクトシティ浜松(静岡県浜松市)
    • Related Report
      2016 Research-status Report
  • [Presentation] Evaluation of alteration in intracellular Ca2+ mobilization with MaxiK channels in the endoplasmic reticulum2016

    • Author(s)
      村田喜理、風間逸郎、丸山芳夫
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2020-03-30  

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