| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
ATP is now recognized as a ubiquitous extracellular messenger in whole body. The physiological roles of ATP release are diverse and its releasing mechanisms have a wide variety. We had developed a real-time ATP luminescence imaging system using luciferin- luciferase bio-luminescence with simultaneous transmission imaging using infrared light under an upright microscope or a macro-view microscope. This imaging system allows us to find differences of pattern and kinetics of the ATP release, which shows various ATP release mechanisms, in keratinocytes, mammary epithelial cells and a lung tissue. These findings demonstrate a diversity of the roles and mechanisms of the ATP release and confirm an effectiveness of the real-time imaging of ATP release.
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