Intracellular M1-muscarinic acetylcholine receptors in central nervous system

• Project/Area Number: 15K08250
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Kanazawa Medical University
• Principal Investigator: MURAMATSU Ikunobu 金沢医科大学, 医学部, 客員教授 (10111965)
• Co-Investigator(Kenkyū-buntansha): 西尾 眞友 金沢医科大学, 医学部, 教授 (80156041) ; 益岡 尚由 金沢医科大学, 医学部, 准教授 (80509307)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ムスカリン受容体 / アセチルコリン / コリン伝達機構 / トランスポーター / 記憶・学習 / アセチルコリントランスポーター / 取り込み / シナプス / ラット脳 / 細胞内受容体 / 細胞内分布

Outline of Final Research Achievements

M1-muscarinic acetylcholine receptors (M1-mAChRs) occur in not only cell surface but also intracelluar sites in CNS neurons and are associated with long term potentiation (LTP) in hippocampus, which has been known to be related to learning and memory. Intracellular M1-mAChRs are activated by ACh, which is transported to postsynaptic neurons though putative ACh transporter. Synaptic ACh concentations are regulated by ACh esterase, ACh transporter and presynaptic M2/M3 autoreceptors. The present study reveals a new aspect of cholinergic transmission in CNS.

Research Products

• [Journal Article] Regulation of synaptic acetylcholine concentrations by acetylcholine transport in rat striatal cholinergic transmission (2017)
  • Author(s): Muramatsu Ikunobu、Uwada Junsuke、Masuoka Takayoshi、Yoshiki Hatsumi、Sada Kiyonao、Lee Kung-Shing、Nishio Matomo、Ishibashi Takaharu、Taniguchi Takanobu
  • Journal Title: Journal of Neurochemistry Volume: 143 Issue: 1 Pages: 76-86
  • DOI: 10.1111/jnc.14127
  • Peer Reviewed
• [Journal Article] TRPA1 channels modify TRPV1-mediated current responses in dorsal root ganglion neurons (2017)
  • Author(s): Takayoshi Masuoka, Makiko Kudo, Yuka Yamashita, Junko Yoshida, Noriko Imaizumi, Ikunobu Muramatsu, Matomo Nishio, Takaharu Ishibashi.
  • Journal Title: Frontiers in Physiology Volume: 8 Pages: 272-272
  • DOI: 10.3389/fphys.2017.00272
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Activation of muscarinic receptors prevents TNF-α-mediated intestinal epithelial barrier disruption through p38 MAPK (2017)
  • Author(s): Uwada Junsuke、Yazawa Takashi、Islam Md Tariqul、Khan Md Rafiqul Islam、Krug Susanne M.、Fromm Michael、Karaki Shin-ichiro、Suzuki Yuichi、Kuwahara Atsukazu、Yoshiki Hatsumi、Sada Kiyonao、Muramatsu Ikunobu、Taniguchi Takanobu
  • Journal Title: Cellular Signalling Volume: 35 Pages: 188-196
  • DOI: 10.1016/j.cellsig.2017.04.007
  • NAID: 120006491645
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pharmacological evidence of specific acetylcholine transport in rat cerebral cortex and other brain regions. (2016)
  • Author(s): Ikunobu Muramatsu, Hatsumi Yoshiki, Junsuke Uwada, Takayoshi Masuoka, Kiyonao Sada, Takanobu Taniguchi, Matomo Nishio.
  • Journal Title: Journal of Neurochemistry Volume: 139 Issue: 4 Pages: 566-575
  • DOI: 10.1111/jnc.13843
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Evidence for conservation of the calcitonin superfamily and activity-regulating mechanisms in the basal chordate Branchiostoma floridae: insight into the molecular and functional evolution in chordates. (2016)
  • Author(s): Sekiguchi, T., Kuwasako, K., Ogasawara, M., Takahashi, H., Matsubara, S., Osugi, T., Muramatsu, I., Sasayama, Y., Suzuki, N., and Satake, H.
  • Journal Title: J. Biol. Chem. Volume: 291 Issue: 5 Pages: 2345-2356
  • DOI: 10.1074/jbc.m115.664003
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Long-term activation of group I metabotropic glutamate receptors increases functional TRPV1-expressing neurons in mouse dorsal root ganglia (2016)
  • Author(s): Takayoshi Masuoka* , Makiko Kudo, Junko Yoshida, Takaharu Ishibashi, Ikunobu Muramatsu, Nobuo Kato, Noriko Imaizumi and Matomo Nishio
  • Journal Title: Front. Cell. Neurosci. Volume: 10 Pages: 79-79
  • DOI: 10.3389/fncel.2016.00079
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Activation of muscarinic cholinoceptor ameliorates tumor necrosis factor-a-induced barrier dysfunction in intestinal epithelial cells. (2015)
  • Author(s): Khan MR, Uwada J, Yazawa T, Islam MT, Krug SM, Fromm M, Karaki S, Suzuki Y, Kuwahara A, Yoshiki H, Sada K, Muramatsu I, Anisuzzaman AS and Taniguchi T.
  • Journal Title: FEBS Lett. Volume: 589 Issue: 23 Pages: 3640-3647
  • DOI: 10.1016/j.febslet.2015.10.029
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Book] Musucarini Receptor: From Structure to Animal Models (2015)
  • Author(s): Ikunobu Muramatsu, Hatsumi Yoshiki, Kiyonao Sada, Junsuke Uwada, Tananobu Taniguchi,
  • Total Pages: 287
  • Publisher: Springer