Long IRBIT regulates cell migration through modulating bicarbonate/chloride anion exchanger activity.
| Project/Area Number |
15K08318
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Showa Pharmaceutical University |
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Principal Investigator |
HAMADA Koichi 昭和薬科大学, 薬学部, 講師 (00343070)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 細胞内ｐH / 細胞移動 / IRBIT / イオントランスポーター / 細胞内ｐH変化 / 転移 / 細胞内ｐＨ / ＩＲＢＩＴ |
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| Outline of Final Research Achievements |
[Purpose] Migration of cancer cells is one of critical parameters in metastasis. We found that more highly metastatic cells showed higher expression of long-IRBIT in both mouse and human melanoma cell lines, suggesting the potential role of long-IRBIT in metastasis. To examine the possibility, we searched for long-IRBIT binding proteins in highly metastatic melanoma cells, and tried to clarify the function of long-IRBIT and its binding protein in cell migration. [Result] A bicarbonate/chloride anion exchanger is identified as a binding protein of Long-IRBIT. Each melanoma cell knocked down for Long-IRBIT or the anion exchanger showed drastic decrease in cell migration ability. Activity of the anion exchanger is significantly reduced in Long-IRBIT knockdown cells. These results suggest that long-IRBIT positively modulates activity of the anion exchanger causing enhancement of cell migration.
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Report
(4 results)
Research Products
(14 results)
