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Development of novel diagnostic markers for low grade urothelial cancer in urine cytology

Research Project

Project/Area Number 15K08380
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Human pathology
Research InstitutionNagasaki University

Principal Investigator

MATSUDA Katsuya  長崎大学, 原爆後障害医療研究所, 助教 (20380967)

Co-Investigator(Kenkyū-buntansha) 中島 正洋  長崎大学, 原爆後障害医療研究所, 教授 (50284683)
Research Collaborator KAWASAKI Tatsuhiko  
ISEKI Masachika  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords尿路上皮癌 / ゲノム不安定性 / DNA損傷応答 / 53BP1 / 尿路上皮腫瘍 / 鑑別診断 / DNA損傷応答分子 / 尿細胞診 / 新規鑑別診断マーカー / 53BP1発現 / 蛍光免疫組織化学
Outline of Final Research Achievements

In this study, ten cases of normal urotheliums, urothelial papillomas, low-grade urothelial carcinoma (LGUC), and high-grade urothelial carcinoma (HGUC), respectively, subjected to double-labelled fluorescence immunohistochemistry of 53BP1 and Ki67. Genome instability (GIN) in urothelial carcinoma was also analysed by FISH. GIN by FISH was absent in normal urotheliums and papillomas, but was 40% and 100% in LGUC and HGUC, respectively. Presence of abnormal 53BP1 was1.5% and 26.1% in papillomas and LGUC, respectively, and distinguished LGUC from papilloma with 90% sensitivity and 95% specificity. Furthermore, co-localization of abnormal 53BP1 and Ki67 was significantly increased in HGUC than in LGUC, distinguishing HGUC from LGUC with 80% sensitivity and 100% specificity. 53BP1 expression analysis in UCs may represent GIN. Immunofluorescence study of 53BP1 in combination with Ki67 may be useful in diagnosing urothelial neoplasms.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2018 2017 2016 2015 Other

All Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (1 results)

  • [Presentation] 膀胱尿路上皮腫瘍での DNA損傷応答分子53BP1発現解析の意義2018

    • Author(s)
      松田勝也、川崎辰彦、井関充及、中島正洋
    • Organizer
      第106回日本病理学会総会(春期大会)
    • Place of Presentation
      京王プラザホテル(東京都新宿区)
    • Year and Date
      2018-04-27
    • Related Report
      2016 Research-status Report
  • [Presentation] 尿路上皮腫瘍でのDNA損傷応答分子53BP1発現解析の意義2017

    • Author(s)
      松田勝也
    • Organizer
      第16回 泌尿器細胞診カンファレンス(別府カンファレンス)in長崎
    • Place of Presentation
      長崎にっしょうかん(長崎県長崎市)
    • Year and Date
      2017-02-25
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 膀胱尿路上皮腫瘍でのDNA損傷応答分子53BP1発現解析の意義2017

    • Author(s)
      松田勝也,川崎辰彦,井関充及,中島正洋
    • Organizer
      第106回日本病理学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Significance of p53-binding protein 1-Nuclear Expression in urothelial tumours: Implication of DNA damage response in association with tumour grades2017

    • Author(s)
      Katsuya Matsuda, Tatsuhiko Kawasaki, Yuko Akazawa, Yuhmi Hasegawa, Masachiko Iseki, Masahiro Nakashima
    • Organizer
      29th European Congress of Pathology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DNA損傷応答分子53BP1の腫瘍組織における発現の意義~病理組織細胞診断への応用に向けて~2016

    • Author(s)
      松田勝也
    • Organizer
      革新的ながん治療・予防研究ユニット第1回TR研究懇談会
    • Place of Presentation
      長崎大学(長崎県・長崎市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] DNA損傷応答分子53BP1の腫瘍組織での発現の意義と細胞診への応用にむけて2015

    • Author(s)
      松田勝也、川崎辰彦、大坪竜太、伊東正博、井関充及、中島正洋
    • Organizer
      第54回日本臨床細胞学会秋期大会
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2015-11-21
    • Related Report
      2015 Research-status Report
    • Invited
  • [Remarks] 長崎大学原爆後障害医療研究所 腫瘍・診断病理学ホームページ 研究内容ページ

    • URL

      http://www-sdc.med.nagasaki-u.ac.jp/pathology/research/index.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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