Development of animal models for study on persistent infection with pancreatropic and cardiotropic picornaviruses
Project/Area Number |
15K08511
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Virology
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Research Institution | National Institute of Infectious Diseases |
Principal Investigator |
Nagata Noriyo 国立感染症研究所, 感染病理部, 室長 (30270648)
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Research Collaborator |
Yamada S.
Hagiwara K.
Miyazaki M.
|
Project Period (FY) |
2015-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | ピコルナウイルス / 膵親和ウイルス / 筋親和ウイルス / 持続感染 / 動物モデル / 膵親和性ウイルス / 筋親和性ウイルス |
Outline of Final Research Achievements |
Coxsackievirus B infection causes acute/chronic diseases such as diabetes and myocarditis; however, the pathogenesis is unclear. Here, we developed an immunodeficient mouse model of persistent infection with recent CVB2 isolates. Immunodeficient mice received CVB2 isolates via the intravenous route and showed transient body weight loss, but survived more than three months. Infectious virus was detected in feces and sera. Histopathological examination revealed that pancreatitis and myocarditis were prolonged in these animals. This animal model will increase our understanding of the pathogenesis of persistent CVB infection.
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Academic Significance and Societal Importance of the Research Achievements |
コクサッキーウイルスB群は慢性あるいは急性の糖尿病や心筋炎を引き起こすことが知られているが、その病態病理は不明のままである。我々は、最近分離されたコクサッキーウイルスB2と免疫不全マウスを利用し、持続感染モデルを作出した。この動物モデルを応用することにより、これらのウイルスによって引き起こされる慢性疾患や持続感染症の発症メカニズムの理解や治療法を検討することが可能となる。
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Report
(5 results)
Research Products
(16 results)
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[Journal Article] Neuropathogenicity of Two Saffold Virus Type 3 Isolates in Mouse Models.2016
Author(s)
Kotani O, Naeem A, Suzuki T, Iwata-Yoshikawa N, Sato Y, Nakajima N, Hosomi T, Tsukagoshi H, Kozawa K, Hasegawa H, Taguchi F, Shimizu H, Nagata N.
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Journal Title
PLOS One
Volume: 11
Issue: 2
Pages: 1-29
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Development of a flaccid paralysis mouse model after infection with Enterovirus D682018
Author(s)
Miyazaki, M, Saito, H, Shibata, C, Doan, YH, Arao, Y, Iwata-Yoshikawa, N, Hasegawa, H, Shimizu H, Nagata N.
Organizer
EUROPIC 2018, 20th International conference on picornaviruses
Related Report
Int'l Joint Research
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[Presentation] Cis-allosteric regulation of the interaction surfaces of Enterovirus A71 capsid protein by the VP1 single amino acid residue at position 1452018
Author(s)
Kotani, O, Yokoyama, M, Fujii, K, Kobayashi, K, Nagata, N, Shimizu, H, Koike, S, Sato, H.
Organizer
EUROPIC 2018, 20th International conference on picornaviruses
Related Report
Int'l Joint Research
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