Discovery of novel treatment strategy for aortic calcification through CT imaging and metabolome analysis

• Project/Area Number: 15K08614
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kobe University
• Principal Investigator: Takaya Tomofumi 神戸大学, 医学研究科, 客員准教授 (60714665)
• Co-Investigator(Kenkyū-buntansha): 篠原 正和 神戸大学, 医学研究科, 准教授 (80437483)
• Co-Investigator(Renkei-kenkyūsha): TOH Ryushi 神戸大学, 大学院医学研究科 内科系講座 立証検査医学分野, 特命准教授 (50379418) ; SHINKE Toshiro 昭和大学, 循環器内科, 教授 (60379419) ; ISHIDA Tatsuro 神戸大学, 大学院医学研究科 地域社会医学・健康科学講座 地域医療ネットワーク学分野, 特命教授 (00379413) ; HIRATA Ken-ichi 神戸大学, 大学院医学研究科 内科学講座 循環器内科学分野, 教授 (20283880)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 大動脈石灰化病変 / 画像診断 / メタボローム解析 / 石灰化病変 / メタボローム

Outline of Final Research Achievements

Establishment of comprehensive method for quantification of calcified lesions in aorta: using CT imaging, we developed widely applicable methodology which easily enables us to re-construct three dimensional(3D) image of calcified lesions in aorta. This method allows us to evaluate global and local quantification of calcified lesions, which provides useful information for cardiovascular physicians and cardiovascular surgeons.
Serum metabolite profiling which reflects calcified lesion volume in aorta: hydrophilic small molecule metabolites in serum samples from patients who received aortic CT imaging were comprehensively analyzed with gas chromatography mass spectrometry (GC/MS). Through GC/MS based metabolome analysis, we found five metabolites showed statistically significant correlation with calcified lesion volume in aorta. We propose that the mathematical function with these five metabolites could be used as quantitative biomarker which reflects calcified lesion volume in aorta.

Research Products

• [Journal Article] The impact of serum trans fatty acids concentration on plaque vulnerability in patients with coronary artery disease: Assessment via optical coherence tomography (2017)
  • Author(s): Nagasawa Yoshinori、Shinke Toshiro、Toh Ryuji、Ishida Tatsuro、Otake Hiromasa、Takaya Tomofumi、Sugiyama Daisuke、Terashita Daisuke、Tahara Natsuko、Shinkura Yuto、Uzu Kenzo、Kashiwagi Daiji、Kuroda Koji、Nagano Yuichiro、Yamamoto Hiroyuki、Yanaka Kenichi、Tsukiyama Yoshiro、Hirata Ken-ichi
  • Journal Title: Atherosclerosis Volume: 265 Pages: 312-317
  • DOI: 10.1016/j.atherosclerosis.2017.06.922
  • NAID: 120006377646
  • Peer Reviewed
• [Journal Article] Morphological and pharmacological determinants of peri-procedural myocardial infarction following elective stent implantation: Optical coherence tomography sub-analysis of the PRASFIT-Elective study (2017)
  • Author(s): Uzu Kenzo、Shinke Toshiro、Otake Hiromasa、Takaya Tomofumi、Osue Tsuyoshi、Iwasaki Masamichi、Kinutani Hiroto、Konishi Akihide、Kuroda Masaru、Takahashi Hachidai、Terashita Daisuke、Hirata Ken-ichi、Saito Shigeru、Nakamura Masato、Shite Junya、Akasaka Takashi
  • Journal Title: Journal of Cardiology Volume: 70 Issue: 6 Pages: 545-552
  • DOI: 10.1016/j.jjcc.2017.05.002
  • Peer Reviewed
• [Journal Article] The association between wedging of the aorta and cardiac structural anatomy as revealed using multidetector-row computed tomography (2017)
  • Author(s): Mori Shumpei、Anderson Robert H.、Takaya Tomofumi、Toba Takayoshi、Ito Tatsuro、Fujiwara Sei、Watanabe Yoshiaki、Nishii Tatsuya、Kono Atsushi K.、Hirata Ken-ichi
  • Journal Title: Journal of Anatomy Volume: 231 Issue: 1 Pages: 110-120
  • DOI: 10.1111/joa.12611
  • Peer Reviewed
• [Journal Article] Compression of the Right Ventricular Outflow Tract due to Straight Back Syndrome Clarified by Low-dose Dual-source Computed Tomography. (2016)
  • Author(s): Hasegawa K, Takaya T, Mori S, Ito T, Fujiwara S, Nishii T, K Kono A, Shimoura H, Tanaka H, Hirata KI.
  • Journal Title: Intern Med. Volume: 55 Pages: 3279-3283
  • NAID: 130005281970
  • Peer Reviewed
• [Journal Article] Serum phosphate is an independent predictor of the total aortic calcification volume in non-hemodialysis patients undergoing cardiovascular surgery. (2016)
  • Author(s): Kinugasa M, Mori S, Takaya T, Ito T, Tanaka H, Satomi-Kobayashi S, Fujiwara S, Nishii T, Kono AK, Okita Y, Hirata K.
  • Journal Title: J Cardiol. Volume: 68 Issue: 4 Pages: 308-15
  • DOI: 10.1016/j.jjcc.2015.10.005
  • Peer Reviewed
• [Journal Article] Clinical cardiac structural anatomy reconstructed within the cardiac contour using multidetector-row computed tomography: Left ventricular outflow tract. (2016)
  • Author(s): Mori S, Fukuzawa K, Takaya T, Takamine S, Ito T, Fujiwara S, Nishii T, Kono AK, Yoshida A, Hirata K.
  • Journal Title: Clin Anat. Volume: 29 Issue: 3 Pages: 353-63
  • DOI: 10.1002/ca.22547
  • Peer Reviewed
• [Journal Article] Clinical cardiac structural anatomy reconstructed within the cardiac contour using multidetector-row computed tomography: The arrangement and location of the cardiac valves. (2016)
  • Author(s): Mori S, Fukuzawa K, Takaya T, Takamine S, Ito T, Fujiwara S, Nishii T, Kono AK, Yoshida A, Hirata K.
  • Journal Title: Clin Anat. Volume: 29 Issue: 3 Pages: 364-70
  • DOI: 10.1002/ca.22549
  • Peer Reviewed
• [Journal Article] Clinical cardiac structural anatomy reconstructed within the cardiac contour using multidetector-row computed tomography: Atrial septum and ventricular septum. (2016)
  • Author(s): Mori S, Fukuzawa K, Takaya T, Takamine S, Ito T, Fujiwara S, Nishii T, Kono AK, Yoshida A, Hirata K.
  • Journal Title: Clin Anat Volume: 29 Issue: 3 Pages: 342-52
  • DOI: 10.1002/ca.22546
  • Peer Reviewed
• [Journal Article] Clinical structural anatomy of the inferior pyramidal space reconstructed from the living heart: Three-dimensional visualization using multidetector-row computed tomography. (2015)
  • Author(s): Mori S, Nishii T, Takaya T, Kashio K, Kasamatsu A, Takamine S, Ito T, Fujiwara S, Kono AK, Hirata K.
  • Journal Title: Clin Anat. Volume: 28 Pages: 878-87
  • Peer Reviewed
• [Journal Article] Three-dimensional quantification and visualization of aortic calcification by multidetector-row computed tomography: a simple approach using a volume-rendering method. (2015)
  • Author(s): Mori S, Takaya T, Kinugasa M, Ito T, Takamine S, Fujiwara S, Nishii T, Kono AK, Inoue T, Satomi-Kobayashi S, Rikitake Y, Okita Y, Hirata K.
  • Journal Title: Atherosclerosis. Volume: 239 Pages: 622-8
  • Peer Reviewed
• [Journal Article] Serum phosphate is an independent predictor of the total aortic calcification volume in non-hemodialysis patients undergoing cardiovascular surgery. (2015)
  • Author(s): Kinugasa M, Mori S, Takaya T, Ito T, Tanaka H, Satomi-Kobayashi S, Fujiwara S, Nishii T, Kono AK, Okita Y, Hirata KI.
  • Journal Title: J Cardiol. Volume: S0914-5087 Pages: 00330-5
  • Peer Reviewed
• [Presentation] Diagnostic performance of a solid-state dedicated cardiac gamma camera, D-SPECT, in the detectin of ischemic heart disease (2016)
  • Author(s): 笠松朗、高谷具史、樫尾和洋、伊藤達郎、森俊平、藤原征、平田健一
  • Organizer: 第80回日本循環器学会学術集会
  • Place of Presentation: 仙台
  • Year and Date: 2016-03-18
• [Presentation] Lung Iodine Map Reconstructed by Dual-energy Computed Tomography Predicts Hemodynamic Status of Patients with Chronic Thromboembolic Pulmonary Hypertension (2016)
  • Author(s): Shumpei Mori, Tomofumi Takaya, Tatsuya Nishii, Yoshiaki Watanabe, Akira Kasamatsu, Kazuhiro Kashio, Tatsuro Ito, Takayoshi Toba, Sei Fujiwara, Kazuhiko Nakayama, Atsushi K Kono, Noriaki Emoto, Ken-ichi Hirata
  • Organizer: 第80回日本循環器学会学術集会
  • Place of Presentation: 仙台
  • Year and Date: 2016-03-18
• [Presentation] "Myocardial extracellular volume fraction quantified using dual-energy cardiac CT correlates with the defect score obtained using nuclear myocardial perfusion imaging " (2015)
  • Author(s): "Tatsuro Ito, Atsushi Kono, Tatsuya Nishii, Akira Kasamatsu, Kazuhiro Kashio, Shumpei Mori, Tomofumi Takaya, Sei Fujiwara, Satoru Takahashi, Ken-ichi Hirata "
  • Organizer: RADIOLOGICAL SOCIETY OF NORTH AMERICA 101st Scientific Assembly and Annual Meeting
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-11-29
  • Int'l Joint Research
• [Presentation] Myocardial extracellular volume fraction measured using dual-energy cardiac CT in patients with old myocardial infarction: A comparison with nuclear myocardial perfusion imaging (2015)
  • Author(s): Tatsuro Ito, Atsushi Kono, Tatsuya Nishii, Kazuhiro Kashio, Akira Kasamatsu, Sachiko Takamine, Shumpei Mori, Tomofumi Takaya, Sei Fujiwara, Ken-ichi Hirata
  • Organizer: Society of Cardiovascular Computed Tomograhy 2015 Annual scientific Meeting
  • Place of Presentation: Las Vegas, USA
  • Year and Date: 2015-07-16
  • Int'l Joint Research
• [Presentation] Myocardial Extracellular Volume Fraction of Interventricular Septum Correlated With Body Size: Dual-Energy Computed Tomographic Study in Normal, Healthy Subjects (2015)
  • Author(s): Tatsuya Nishii, Atsushi K Kono, Niroyuki Negi, Shumpei Mori, Tatsuro Ito, Sachiko Takamine, Tomofumi Takaya, Sei Fujiwara, Ken-ichi Hirata, Kazuro Sugimura
  • Organizer: Society of Cardiovascular Computed Tomograhy 2015 Annual scientific Meeting
  • Place of Presentation: Las Vegas, USA
  • Year and Date: 2015-07-16
  • Int'l Joint Research
• [Presentation] Low Contrast Agent and Radiation Dose Protocol for Coronary CT Angiography using 4rd Generation Dual-source CT (2015)
  • Author(s): Atsushi Kono, Tatsuya Nishii, Kazuhiro Kashio, Akira Kasamatsu, Sachiko Takamine, Tatsuro Ito, Shumpei Mori,Tomofumi Takaya, Sei Fujiwara, Satoru Takahashi, Ken-ichi Hirata, Kazuro Sugimura
  • Organizer: Society of Cardiovascular Computed Tomograhy 2015 Annual scientific Meeting
  • Place of Presentation: Las Vegas, USA
  • Year and Date: 2015-07-16
  • Int'l Joint Research
• [Remarks] 神戸大学大学院医学研究科 循環器内科学分野
  • URL: http://www.med.kobe-u.ac.jp/im1/index.html