Development of micro blood clinical examination with deepening of homogeneous assay by magnetic detection

• Project/Area Number: 15K08632
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Nagasaki International University
• Principal Investigator: Kuma Hiroyuki 長崎国際大学, 薬学部, 准教授 (40435136)
• Co-Investigator(Kenkyū-buntansha): 濱崎 直孝 長崎国際大学, 薬学部, 客員教授 (00091265)
• Research Collaborator: TSUKAMOTO Akira 超伝導センシング技術研究組合, 研究員
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 磁気マーカー / 免疫検査システム / 抗原抗体反応 / ホモジニアスアッセイ / SQUIDセンサー / 免疫検査

Outline of Final Research Achievements

We have developed the magnetic immunoassay system using magnetic markers consist of Fe3O4 beads and antibody molecules, and SQUID magnetic sensor by constructing a simple, and highly sensitive magnetic homogeneous assay with deepening their components. We have also demonstrated that the ability for our method through the experiments using our prototype machine without the washing step of B / F (Bound / Free) separation.

Research Products

• [Journal Article] Genotyping analysis of protein S-Tokushima (K196E) and the involvement of protein S antigen and activity in patients with recurrent pregnancy loss (2017)
  • Author(s): Matsukawa Y, Asano E, Tsuda T, Kuma H, Kitaori T, Katano K, Ozaki Y, Sugiura- Ogasawara M
  • Journal Title: Eur J Obstet Gynecol Reprod Biol Volume: 211 Pages: 90-97
  • DOI: 10.1016/j.ejogrb.2017.01.056
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of Band3 in the efflux of sphingosine 1-phosphate from erythrocytes (2017)
  • Author(s): Kurano M, Nishikawa M, Kuma H, Jona M, Yatomi Y.
  • Journal Title: PLOS ONE Volume: 12 Issue: 5 Pages: e0177543-e0177543
  • DOI: 10.1371/journal.pone.0177543
  • Peer Reviewed / Open Access
• [Journal Article] Multianalyte Conventional Reference Material (MacRM): A Useful Tool for Nationwide Standardization of Laboratory Measurements for Medical Care-A Model Study in Japan (2016)
  • Author(s): Shinohara K, Hamasaki N, Takagi Y, Yatomi Y, Kikuchi H, Hosogaya S, Kawai Y, Miyachi H, Kaneko K, Miyajima Y, Matsumoto H, Yamamoto Y, Iwagami M, Osawa S, Umeda M, Koide H, Yoshimura D, Kato H
  • Journal Title: Clinical Chemistry Volume: 62 Issue: 2 Pages: 392-406
  • DOI: 10.1373/clinchem.2015.245621
  • Peer Reviewed / Open Access
• [Journal Article] Crystal structure of the anion exchanger domain of human erythrocyte band 3 (2015)
  • Author(s): Arakawa T, Kobayashi-Yurugi T, Alguel Y, Iwanari H, Hatae H, Iwata M, Abe Y, Hino T, Ikeda-Suno C, Kuma H, Kang D, Murata T, Hamakubo T, Cameron AD, Kobayashi T, Hamasaki N, Iwata S
  • Journal Title: Science Volume: 350 Issue: 6261 Pages: 680-684
  • DOI: 10.1126/science.aaa4335
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 改良型磁気検出システムを用いたスギ花粉抗原の検出法 (2018)
  • Author(s): 鍬先 玲奈，髙安 結，波多江 日成子，円福 敬二，隈 博幸
  • Organizer: 日本薬学会第138年会
• [Presentation] 月経周期と凝固制御因子活性の相関解析 (2018)
  • Author(s): 川原 里歩，竹本 有里，西尾 霞，原口 晴圭，高崎 伸也，隈 博幸，濱崎 直孝，波多江 日成子
  • Organizer: 日本薬学会第138年会
• [Presentation] ダビガトランのプロテインS比活性測定に与える影響 (2017)
  • Author(s): 中島明日菜，明吉祥花，市山朋樹，高崎伸也，隈博幸
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 仙台国際会議場
  • Year and Date: 2017-03-24
• [Presentation] 磁気的免疫検査システムを用いたスギ花粉抗原の検出 (2017)
  • Author(s): 髙安結，東牧里奈，円福敬二，隈博幸
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 仙台国際会議場
  • Year and Date: 2017-03-24
• [Presentation] 新しいVTE：プロテインS活性、及びプロテインS比活性測定 (2017)
  • Author(s): 隈 博幸
  • Organizer: 第39回日本血栓止血学会学術集会
• [Presentation] プロテインS比活性測定 (2016)
  • Author(s): 隈博幸，太田一寿，浦田美秩代，隈美智代，濱崎直孝
  • Organizer: 第63回日本臨床検査医学会学術集会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2016-09-01
• [Remarks] 磁気検出法を用いた免疫検査システムの開発（研究室HP）
  • URL: http://www.niu.ac.jp/~pharm1/lab/cclm/immunoassay.html
• [Remarks] 長崎国際大学薬学部臨床検査学
  • URL: http://www.niu.ac.jp/~pharm1/lab/cclm/immunoassay.html
• [Remarks] 長崎国際大学薬学部臨床検査学研究室
  • URL: http://www.niu.ac.jp/~pharm1/lab/cclm/immunoassay.html