Exploration new biomarkers based on HTLV-1 carrier clone analysis
| Project/Area Number |
15K08647
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
HASEGAWA Hiroo 長崎大学, 病院(医学系), 講師 (00398166)
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| Co-Investigator(Kenkyū-buntansha) |
波多 智子 長崎大学, 原爆後障害医療研究所, 准教授 (10346968)
宮崎 泰司 長崎大学, 原爆後障害医療研究所, 教授 (40304943)
柳原 克紀 長崎大学, 医歯薬学総合研究科(医学系), 教授 (40315239)
今泉 芳孝 長崎大学, 病院(医学系), 講師 (40404305)
佐々木 大介 長崎大学, 病院(医学系), 技術職員 (90624784)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | HTLV-1 / 細胞株 / 分子標的 / 表面抗原 / 遺伝子発現解析 / バイオマーカー |
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| Outline of Final Research Achievements |
In this study, we clarified the hematological and immunological characteristics of HTLV-1 infected cells in carriers using 10 cell lines established from HTLV-1 carriers in our laboratory. As a comparative control, 10 smoldering ATL cell lines and 7 acute type ATL cell lines, also established in our laboratory, are used. First, we performed surface antigen analysis of various CD antigens, cDNA microarray analysis, and miRNA expression analysis using these cell lines. Then, Southern blot analysis for HTLV-1, quantitative RT-PCR test for HTLV-1 TAX and HBZ, and flow cytometric analysis were performed using carrier cell lines. Thus far, we analyzed the results of flow cytometry analysis and microarray analysis.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Proviral features of human T cell leukemia virus type 1 in carriers with indeterminate western blot analysis results.2017
Author(s)
Kuramitsu M, Sekizuka T, Yamochi T, Firouzi S, Sato T, Umeki K, Sasaki D, Hasegawa H, Kubota R, Sobata R, Matsumoto C, Kaneko N, Momose H, Araki K, Saito M, Nosaka K, Utsunomiya A, Koh K, Ogata M, Uchimaru K, Iwanaga M, Sagara Y, Yamano Y, et al.
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Journal Title
J Clin Microbiol.
Volume: 55(9)
Issue: 9
Pages: 2838-2849
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Induction of apoptosis by HBI-8000 in adult T-cell leukemia/lymphoma is associated with activation of Bim and NLRP3.2016
Author(s)
Hasegawa H, Bissonnette RP, Gillings M, Sasaki D, Taniguchi H, Kitanosono H, Tsuruda K, Kosai K, Uno N, Morinaga Y, Imaizumi Y, Miyazaki Y, Yanagihara K.
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Journal Title
Cancer Sci
Volume: 107
Issue: 8
Pages: 1124-33
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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