Elucidation of mechanism of itch transmission to spinal cord using in vivo patch clamp and three-dimensional electron microscope.
Project/Area Number |
15K08667
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Pain science
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Research Institution | University of Toyama |
Principal Investigator |
Uta Daisuke 富山大学, 大学院医学薬学研究部(薬学), 助教 (70598416)
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Co-Investigator(Kenkyū-buntansha) |
安東 嗣修 富山大学, 大学院医学薬学研究部(薬学), 准教授 (50333498)
高浪 景子 国立遺伝学研究所, 遺伝形質研究系, 助教 (70578830)
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Research Collaborator |
SAKAMOTO hirotaka
Kiguchi norikazu
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Project Period (FY) |
2015-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | 痒み / 痛み / in vivo patch clamp / spinal dorsal horn / 5-HT / GRP / electrophysiology / itch / pain / spinal cord / 脊髄後角 / Nppb / in vivoパッチクランプ / 興奮性シナプス応答 |
Outline of Final Research Achievements |
The mechanism for the itch transmission in the central nervous system remains unclear. The present study was designed to analyze pruritic synaptic responses evoked in spinal dorsal horn and cortex neurons of rodents by using an in vivo whole-cell patch-clamp and extracellular recording technique. The present main findings are that pruriceptive itch-sensitive afferent fibers evoke excitatory synaptic responses mainly onto delayed firing and sustained repetitive firing type neurons which are morphologically classified as the excitatory interneurons.
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Academic Significance and Societal Importance of the Research Achievements |
痒みは非常に不快な感覚であり、特にアトピー性皮膚炎など慢性そう痒は既存薬物では効果を示さないことが多く患者のQOLを著しく低下させる。しかし、痒みの情報伝達機構は不明な点が多い。申請課題の研究成果により、痒みの情報伝達機構や、痒み受容ニューロンの特徴が明らかになった。このことは、今後慢性そう痒モデル動物を用いることで病態時の解析にも非常に役立つ成果である。更に、得られた成果は新規治療法、治療薬を開発するうえで重要な情報となりうる。
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Report
(5 results)
Research Products
(132 results)
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[Journal Article] Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish.2016
Author(s)
Sakamoto, T., Yoshiki, M., Takahashi, H., Yoshida, M., Ogino, Y., Ikeuchi, T., Nakamachi, T., Konno, N., Matsuda, K. and Sakamoto, H.
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Journal Title
Scientific Reports
Volume: -
Issue: 1
Pages: 37991-37991
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Errata to “Structure-function correlations of rat trigeminal primary neurons: Emphasis on club-like endings, a vibrissal mechanoreceptor”2016
Author(s)
Tonomura S, Ebara S, Bagdasarian K, Uta D, Ahissar E, Meir I, Lampl I, Kuroda D, Furuta T, Furue H, Kumamoto K.
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Journal Title
Proceedings of the Japan Academy, Series B
Volume: 92
Issue: 2
Pages: 76-76
DOI
NAID
ISSN
0386-2208, 1349-2896
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Observation of Mosquito Skin Penetration and Blood Suction in laboratory animals.2017
Author(s)
Kitada H, Yamamoto H, Takahashi T, Suzuki M, Aoyagi S, Yamamoto S, Hosomi R, Fukunaga K, Uta D, Takazawa T, Hikitsuchi T, Kawajiri Y, Nakayama K.
Organizer
精密工学会秋季大会学術講演会
Related Report
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[Presentation] Investigation on mechanism of mosquito to puncture blood vessel wall using fertilized egg and its application to microneedles.2017
Author(s)
Okuda K, Yamamoto S, Takahashi T, Suzuki M, Aoyagi S, Fukunaga K, Hoshomi R, Takazawa T, Uta D, Hikitsuchi T, Kawajiri Y, Nakayama K.
Organizer
精密工学会秋季大会学術講演会
Related Report
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