Development of minimal-invasive and quantitative analytical method for nitrated DNA damage, and its application to risk assessment of inflammation-related carcinogenesis
| Project/Area Number |
15K08787
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hygiene and public health
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| Research Institution | Suzuka University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大西 志保 鈴鹿医療科学大学, 薬学部, 助教 (80511914)
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| Co-Investigator(Renkei-kenkyūsha) |
MA Ning 鈴鹿医療科学大学, 医療科学研究科, 教授 (30263015)
TABATA Tsutomu 三重大学, 医学研究科, 准教授 (40252358)
IIJIMA Katsunori 秋田大学, 医学研究科, 教授 (60375003)
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| Research Collaborator |
MURATA Mariko 三重大学大学院, 医学研究科, 教授 (10171141)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | がん予防 / 炎症 / DNA損傷 |
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| Outline of Final Research Achievements |
Chronic infection and inflammation have been recognized as important factors for carcinogenesis. Inflammation is estimated to contribute to approximately 25 % of human cancers. It is urgent problem to be solved, the risk assessment in early phase and prevention of inflammation-related carcinogenesis. We have reported that 8-nitroguanine, nitrative DNA damage specifically generated under inflammation, is generated in cancerous site before carcinogenesis. Therefore, 8-nitroguanine is hopeful marker for risk assessment of inflammation-related carcinogenesis. However, it is difficult to analyze 8-nitroguanine with quantitativity and minimal-invasiveness. In this study, we successed to confirm the amount of 8-nitroguanine quantitatively with using high performance liquid chromatography coupled with an electrochemical detector (HPLC-ECD).
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Report
(4 results)
Research Products
(21 results)
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[Presentation] Inflammation-related DNA damage in relation to the expression of cancer stemness markers in human nasopharyngeal carcinoma.2015
Author(s)
Wang S, Ma N, Kawanishi S, Zhao W, Midorikawa K, Hiraku Y, Oikawa S, Zhang Z, Huang G, Murata M
Organizer
SFRR-E/SNFS Meeting Stuttgart 2015: Redox Biology Meets Nutrition
Place of Presentation
University of Hohenheim (Stuttgart, Germany)
Year and Date
2015-09-01
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