| Project/Area Number |
15K09030
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kurume University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
池園 友 久留米大学, 医学部, 助教 (10461419)
古賀 浩徳 久留米大学, 医学部, 教授 (90268855)
増田 篤高 久留米大学, 医学部, 助教 (40647872)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ペプチドハイドロゲル / iPS細胞 / 肝硬変 / 肝再生 / 細胞療法 |
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| Outline of Final Research Achievements |
This study is a fundamental study aiming at the clinical application of liver regeneration therapy using self-assembling peptide hydrogel (PuraMatrix). We investigated whether transplantation of PuraMatrix, which enabled for the first time a three-dimensional environment as a scaffolding material for tissue engineering, mixed with human iPS cells-derived hepatocytes (iHep), endothelial cells (iEC), and equal volumes of iHep and iEC could reduce established liver fibrosis and up-regulate hepatic regeneration in liver cirrhotic model mice. Reduction of liver fibrosis by transplantation of iHep, iEC, and equal volumes of iHep and iEC with and without peptide hydrogel was demonstrated by Mallory's Azan histologic staining and by immunohistochemical analysis for alpha-SMA in CCl4-treated livers.
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