Differentiation of human induced pluripotent stem cells to hepatocyte lineage
| Project/Area Number |
15K09032
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | 独立行政法人国立病院機構 下志津病院(臨床研究部) |
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Principal Investigator |
Tomizawa Minoru 独立行政法人国立病院機構 下志津病院(臨床研究部), 臨床研究部, その他 (90334193)
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| Co-Investigator(Renkei-kenkyūsha) |
OHIRA Miki 千葉県がんセンター(研究所), がんゲノム研究室, 室長 (20311384)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | iPS細胞 / 肝細胞 / ブドウ糖 / アルギニン / 乳酸 / 糖新生 / 尿素サイクル / Coriサイクル / 肝細胞分化 / 解糖系 / 転写因子 / ガラクトース / オルニチン |
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| Outline of Final Research Achievements |
To promote differentiation of human induced pluripotens stem (iPS) cells to hepatocytes, oncostati M, and M50054 were added to a medium without glucose or arginine, and added with galactose and ornithine (hepatocyte differentiation inducer). The expression levels of alpha-feto protein were up-regulated in iPS cells cultured in HDI for 2 days. It was suggested that HDI promoted differentiation of iPS cells to hepatocyte lineage.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Involvement of Wnt pathway in feeder-free culture of human induced pluripotent stem cells2015
Author(s)
Tomizawa M, Shinozaki, F, Motoyoshi, Y, Sugiyama, T, Shigenori Yamamoto, S, Ishige, N
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Journal Title
Mol Med Rep
Volume: 12
Issue: 5
Pages: 6797-6800
DOI
Related Report
Peer Reviewed
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