Study of biomarkers in respiratory disease based on the expression analysis of miRNAs in blood and epithelial lining fluid.

• Project/Area Number: 15K09179
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Kagoshima University
• Principal Investigator: SAMUKAWA Takuya 鹿児島大学, 医歯学域附属病院, 講師 (10363623)
• Co-Investigator(Kenkyū-buntansha): 井上 博雅 鹿児島大学, 医歯学域医学系, 教授 (30264039)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 呼吸器疾患 / バイオマーカー / 気道上皮被覆液 / miRNA / napsin A / BAFF / miRNA発現解析 / 気管支鏡下マイクロサンプリング / 間質性肺炎 / 血液バイオマーカー

Outline of Final Research Achievements

1.Serum B cell-activating factor (BAFF) may be a useful marker for distinguish connective tissue disease associated interstitial lung disease from chronic fibrosing interstitial pneumonia. 2. miRNA-218 on lung squamous cell carcinoma directly regulates oncogenic TDP52 and appears to function as a tumor suppresser through targeting of TDP52. 3.Evaluation of napsin A levels in epithelial lining fluid may be useful for distinguishing primary lung adenocarcinoma.

Research Products

• [Journal Article] Napsin A levels in epithelial lining fluid as a diagnostic biomarker of primary lung adenocarcinoma. (2017)
  • Author(s): Uchida T, Samukawa T, Kumamoto T, Ohshige M, Hatanaka K, Nakamura Y, Mizuno K, Higashimoto I, Sato M, Inoue H.
  • Journal Title: BMC Pulm Med Volume: 17
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of TPD52 by antitumor microRNA-218 suppresses cancer cell migration and invasion in lung squamous cell carcinoma. (2016)
  • Author(s): Kumamoto T, Seki N, Mataki H, Mizuno K, Kamikawaji K, Samukawa T, Koshizuka K, Goto Y, Inoue H.
  • Journal Title: Int J Oncol. Volume: 49 Issue: 5 Pages: 1870-1880
  • DOI: 10.3892/ijo.2016.3690
  • Peer Reviewed / Open Access
• [Journal Article] Interstitial pneumonia induced by bleomycin treatment is exacerbated in Angptl2-deficient mice (2016)
  • Author(s): Motokawa I, Endo M, Terada K, Horiguchi H, Miyata K, Kadomatsu T, Morinaga J, Sugizaki T, Ito T, Araki K, Morioka MS, Manabe I, Samukawa T, Watanabe M, Inoue H, Oike Y.
  • Journal Title: American Journal of Physiology. Lung Cellular and Molecular Physiology Volume: 311 Issue: 4 Pages: 704-713
  • DOI: 10.1152/ajplung.00005.2016
  • Peer Reviewed
• [Journal Article] Serum B cell-activating factor (BAFF) level in connective tissue disease associated interstitial lung disease. (2015)
  • Author(s): Hamada T, Samukawa T, Kumamoto T, Hatanaka K, Tsukuya G, Yamamoto M, Machida K, Watanabe M, Mizuno K, Higashimoto I, Inoue Y, Inoue H.
  • Journal Title: BMC Pulm Med Volume: 15 Issue: 1 Pages: 110-110
  • DOI: 10.1186/s12890-015-0105-0
  • Peer Reviewed / Open Access / Acknowledgement Compliant