| Project/Area Number |
15K09214
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
SUDA TAKAFUMI 浜松医科大学, 医学部, 教授 (30291397)
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| Co-Investigator(Kenkyū-buntansha) |
榎本 紀之 浜松医科大学, 医学部附属病院, 講師 (50436961)
中村 祐太郎 浜松医科大学, 医学部附属病院, 講師 (60436962)
永田 年 浜松医科大学, 医学部, 教授 (90275024)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | ワクチン / 樹状細胞 / 結核 / 細胞内寄生菌 |
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| Outline of Final Research Achievements |
We attempted to develop a novel cell-based vaccine using dendritic cells (DC) and biodegradable polylactic glycolic acid (PLGA)-nanoparticles (PLGA) for intracellular infections, such as tuberculosis. Bone marrow-derived DC pulsed with PLGA, which was coated with ovalbumin protein (OVA) (DC-PLGA/OVA), potently induced a peptide-specific T cell response through efficient cross-presentation in vitro, assessed by peptide-specific T cell proliferation and IFN-gamma production. However, in vivo, immunization with DC-PLGA/OVA failed to elicit a strong peptide-specific T cell response, based on the results of peptide-specific T cell proliferation and the number of peptide-specific T cell determined with tetramer.
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