Proteomics of peripheral exosome identifies novel biomarkers for COPD
| Project/Area Number |
15K09219
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
植田 幸嗣 公益財団法人がん研究会, がんプレシジョン医療研究センター がんオーダーメイド医療開発プロジェクト, プロジェクトリーダー (10509110)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | バイオマーカー / プロテオミクス / エクソソーム / 閉塞性肺疾患 / COPD / proteomics / 喘息 / exosome |
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| Outline of Final Research Achievements |
In spite of massive investment in the discovery of novel biomarkers, there is currently no specific biomarker for COPD. In order to challenge this problem, we focused on exosomes which contain both great deal of information from pathogenic cells and less protein such as albumin by utilizing the most advanced proteomics.
To explore novel biomarkers for inflammation as well as COPD, we utilized acute lung injury model by LPS and emphysema model by elastase. An iTRAQ-Based Proteomic Analysis and subsequent MRM verification were performed. From Quantitative Proteomics, we obtained 1300 proteins. Among them, 64 proteins were up-regulated in emphysema model, 52 proteins in LPS group, and 14 proteins in both groups. Of note, these proteomic profiles reflect their characteristics, respectively. Among 26 proteins from these candidates, 14 proteins were verified by MRM. Using these novel BM candidates, we are further going to validate them in human.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Double deletion of tetraspanins CD9 and CD81 in mice leads to a syndrome resembling accelerated aging2018
Author(s)
Jin Y, Takeda Y, Kondo Y, Tripathi LP, Kang S, Takeshita H, Kuhara H, Maeda Y, Higashiguchi M, Miyake K, Morimura O、Koba T、Hayama Y、Koyama S、Nakanishi K、Iwasaki T、Tetsumoto Si、Tsujino K、Kuroyama M、Iwahori K、Hirata H、Takimoto Ti、Suzuki M、Nagatomo I、Sugimoto K、Fujii Y、Kida H、et al.
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Journal Title
Scientific Reports
Volume: 8
Issue: 1
Pages: 5145-5145
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Classification of idiopathic interstitial pneumonias using anti-myxovirus resistance-protein 1 autoantibody2017
Author(s)
Hamano Y, Kida H, Ihara S, Murakami A, Yanagawa M, Ueda K, Honda O, Tripathi LP, Arai T, Hirose M, Hamasaki T, Yano Y, Kimura T, Kato Y, Takamatsu H, Otsuka T, Minami T, Hirata H, Inoue K, Nagatomo I, Takeda Y, Mori M, Nishikawa H, Mizuguchi K, Kijima T, Kitaichi T, Tomiyama N, Inoue Y, Kumanogoh A
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Journal Title
Scientific Reports
Volume: 23
Issue: 1
Pages: 43201-43201
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Rapid intracranial response to osimertinib, without radiotherapy, in nonsmall cell lung cancer patients harboring the EGFR T790M mutation: Two Case Reports2017
Author(s)
Koba T, Kijima T, Takimoto T, Hirata H, Naito Y, Hamaguchi M, Otsuka T, Kuroyama M, Nagatomo I, Takeda Y, Kida H, Kumanogoh A
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Journal Title
Medicine (Baltimore)
Volume: 96
Issue: 6
Pages: 6087-6087
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Clarifying the biological significance of the CHK2 K373E somatic mutation discovered in The Cancer Genome Atlas database2016
Author(s)
Higashiguchi M, Nagatomo I, Kijima T, Morimura O, Miyake K, Minami T, Koyama S, Hirata H, Iwahori K, Takimoto T, Takeda Y, Kida H, Kumanogoh A
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Journal Title
FEBS letters
Volume: 590
Issue: 23
Pages: 4275-4286
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Epiplakin is a paraneoplastic pemphigus autoantigen and related to bronchiolitis obliterans in Japanese patients.2016
Author(s)
(2)Tsuchisaka A, Numata S, Teye K, Natsuaki Y, Kawakami T, Takeda Y, Wang W, Ishikawa K, Goto M, Koga H, Sogame R, Ishii N, Takamori S, Hoshino T, Brandt O, Pas HH, Fujiwara S, Hashimoto T.
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Journal Title
J Invest Dermatol.
Volume: 136
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Transcriptomics Identify CD9 as a marker of murine IL-10-competent regulatory B cells.2015
Author(s)
(5)Sun J, Wang J, Pefanis E, Chao J, Rothschild G, Tachibana I, Chen JK, Ivanov II, Rabadan R, Takeda Y, Basu U.
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Journal Title
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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