Treatment for kidney disease using fetal membrane-derived mesenchymal stem cells.

• Project/Area Number: 15K09256
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Aichi Medical University (2017-2018); Nagoya University (2015-2016)
• Principal Investigator: Katsuno Takayuki 愛知医科大学, 医学部, 講師 (60642337)
• Co-Investigator(Kenkyū-buntansha): 丸山 彰一 名古屋大学, 医学系研究科, 教授 (10362253)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 再生医療 / 細胞治療 / 間葉系幹細胞 / 腎臓 / 卵膜 / 低血清培養 / 卵膜由来 / 再生医学

Outline of Final Research Achievements

Applicants' previous studies have identified mesenchymal stem cells (MSC) differentiation under conditions where the serum concentration of the culture solution of fetal membrane-derived MSC (FM-MSC) was changed to 2%. However, in the low serum culture method with a serum concentration of 2%, the proliferation ability of the FN-MSCs was attenuated, and the proliferation rate was decreased in passage3. When hFGF (human fibroblast growth factor) was added for the purpose of promoting the growth of MSCs and the culture medium was introduced, an improvement in the growth rate was obtained but the problem that the undifferentiated state was not maintained became clear. The FM- MSC could not give the same result as that of adipose tissue-derived MSC. This study suggested that low serum culture may be dependent on cell source.

Academic Significance and Societal Importance of the Research Achievements

本研究は再生医療における細胞ソースとして、卵膜由来間葉系幹細胞を用いようとする点に特色がある。この際、世界に先駆け開発した低血清培養法を用いる点に独創性がある。さらに卵膜由来間葉系幹細胞を自家細胞移植だけでなく、他家細胞移植にも用いようとする点は学術的な意義がある。卵膜由来間葉系幹細胞の培養法及び治療法の確立が達成できれば、再生医療産業化の促進に期待が持たれる。

Research Products

• [Journal Article] Urinary soluble CD163 level reflects glomerular inflammation in human lupus nephritis. (2016)
  • Author(s): Endo N, Tsuboi N, Furuhashi K, Shi Y, Du Q, Abe T, Hori M, Imaizumi T, Kim H, Katsuno T, Ozaki T, Kosugi T, Matsuo S, Maruyama S.
  • Journal Title: Nephrol Dial Transplant Volume: 31(12) Issue: 12 Pages: 2023-2033
  • DOI: 10.1093/ndt/gfw214
  • NAID: 120006220242
  • Peer Reviewed
• [Journal Article] Transfusion of CD206+ M2 Macrophages Ameliorates Antibody-Mediated Glomerulonephritis in Mice. (2016)
  • Author(s): Du Q, Tsuboi N, Shi Y, Ito S, Sugiyama Y, Furuhashi K, Endo N, Kim H, Katsuno T, Akiyama S, Matsuo S, Isobe KI, Maruyama S.
  • Journal Title: Am J Pathol. Volume: 186 Issue: 12 Pages: 3176-3188
  • DOI: 10.1016/j.ajpath.2016.08.012
  • Peer Reviewed
• [Presentation] UMAN ADIPOSE-DERIVED STROMAL CELLS AMELIORATE CRESCENTIC GLOMERULONEPHRITIS (2017)
  • Author(s): Yuko Shimamura, Akimitsu Kitagawa Yutaka KamimuraYutaka Sugiyama Asuka Horinouchi Takayuki Katsuno,Naotake Tsuboi,and Shoichi Maruyama
  • Organizer: the 18th international vasculitis & ANCA workshop
  • Place of Presentation: 東京大学(東京都文京区）
  • Year and Date: 2017-03-26
• [Presentation] TLR3 Activation Enhances the Renoprotective Effect of Low Serum Cultured Adipose Derived Stromal Cell for Anti-GBM Nephritis (2017)
  • Author(s): Yutaka Kamimura, Naotake Tsuboi, Akimitsu Kitagawa, Takayuki Katsuno, Shoichi Maruyama
  • Organizer: 50th Annual Meeting of the American Society of Nephrology
  • Int'l Joint Research
• [Presentation] HUMAN ADIPOSE-DERIVED STROMAL CELLS AMELIORATE CRESCENTIC GLOMERULONEPHRITIS (2017)
  • Author(s): Yuko Shimamura, Akimitsu Kitagawa Yutaka KamimuraYutaka Sugiyama Asuka Horinouchi Takayuki Katsuno,Naotake Tsuboi,and Shoichi Maruyama
  • Organizer: the 18th international vasculitis & ANCA workshop
  • Int'l Joint Research
• [Presentation] AIRED IMMUNOGLOBULIN-LIKE TYPE2 RECEPTOR ALPHA (PILRA) NEGATIVELY REGULATES MOUSE CRESCENTIC GLOMERULONEPHRITIS (2017)
  • Author(s): Yutaka Sugiyama。Naotake Tsuboi,Yuko Shimamura,Akimitsu Kitagawa, Yutaka Kamimura, Asuka HorinouchiTakayuki Katsuno,and Shoichi Maruyama
  • Organizer: the 18th international vasculitis & ANCA workshop
  • Int'l Joint Research
• [Presentation] anti-GBM diseaseに対する低血清培養脂肪由来幹細胞治療の有効性 (2015)
  • Author(s): 北川 章充、堀之内 明日花、坪井 直毅、松尾 清一、丸山 彰一
  • Organizer: 第58回日本腎臓学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-06-07
• [Book] リウマチ科 (2016)
  • Author(s): 丸山彰一、山口真、勝野敬之、坪井直毅
  • Total Pages: 230
  • Publisher: 科学評論社