| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
The physiological role of serine protease hepsin, which is expressed in various human tissues including liver, spleen, and kidney, is unknown. Previous studies revealed selective increase in bone alkaline phosphatase in hepsin knockout mice. Based on reports showing the inactivation and degradation of the sodium phosphate cotransporter (NaPi-IIa) via cleavage by proteases, we hypothesized that hepsin reduces phosphate reabsorption in renal proximal tubules. We identified rat hepsin expression along the nephron segment and investigated its expression in mice with acute or chronic phosphate overload. Rat hepsin mRNA was predominantly expressed in proximal tubules, whereas lower hepsin mRNA expression levels were detected in distal and collecting duct tubules. Acute and chronic phosphate overload had no effect on mouse hepsin mRNA or hepsin protein expression in renal tubules. These findings suggested that the serine protease hepsin was not involved in phosphate handling in the kidney.
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