Analysis of renal tubular function using siRNA

• Project/Area Number: 15K09299
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Tohoku Medical and Pharmaceutical University (2016-2017); Nihon University (2015)
• Principal Investigator: MORIMOTO Tetsuji 東北医科薬科大学, 医学部, 准教授 (10344657)
• Co-Investigator(Kenkyū-buntansha): 根東 義明 日本大学, 医学部, 教授 (00221250) ; 石毛 美夏 日本大学, 医学部, 講師 (90420950)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 長時間微小単離尿細管灌流法 / 腎尿細管機能解析 / siRNA / 長時間灌流 / 微小単離尿細管灌流法

Outline of Final Research Achievements

The subject of this research was to analyze renal tubular function after delivering siRNA using in vitro microperfusion. We tried to establish an in vitro microperfusion for more than 48 hours. Finally, we succeeded to measure transepithelial voltage in cortical thick ascending limb after about 36 hours of the experiment. If we introduce a disposal chamber to avoid a contamination, we have a good chance to establish an in vitro a long time microperfusion.