Longitudinal analyses of genetic alterations in malignant lymphoma using cell-free DNA: detection of refractory disease related abnormalities and application possibility in clinical trials.
| Project/Area Number |
15K09473
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Fujita Health University |
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Principal Investigator |
Tomita Akihiro 藤田保健衛生大学, 医学部, 准教授 (80378215)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIDA Kenichi 京都大学, 医学部・腫瘍生物学講座 (50738226)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 悪性リンパ腫 / 網羅的遺伝子変異解析 / 末梢血無細胞遊離DNA / リキッドバイオプシー / 血管内大細胞型B細胞リンパ腫 / 免疫回避 / びまん性大細胞型B細胞リンパ腫 / 遺伝子変異 |
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| Outline of Final Research Achievements |
Accumulation of genetic mutations is critical for lymphomatogenesis, disease progression and sensitivity to targeting therapeutics in malignant lymphoma (ML). In this analysis, peripheral blood cell-free DNA (cfDNA) was longitudinally analyzed to reveal correlation with clinical features and to detect tumor specific genetic alterations. Especially in intravascular large B-cell lymphoma, those tumor cells are hardly obtained from biopsy sample, cfDNA was an alternative tumor DNA source to detect specific genetic alterations in comprehensive genetic analyses. Genetic analyses using cfDNA may become an alternative powerful strategy to detect tumor specific genetic abnormalities not only for diagnosis but also longitudinal analyses to predict sensitivity to molecular targeting drugs, even in patients whose tumor cells cannot be obtained easily during their clinical courses.
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Report
(4 results)
Research Products
(49 results)
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[Journal Article] Rearrangement of VPS13B, a causative gene of Cohen syndrome, in a case of RUNX1-RUNX1T1 leukemia with t(8;12;21).2017
Author(s)
Abe A, Yamamoto Y, Katsumi A, Okamoto A, Tokuda M, Inaguma Y, Yamamoto K, Yanada M, Kanie T, Tomita A, Akatsuka Y, Okamoto M, Kameyama T, Mayeda A, Emi N.
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Journal Title
Int J Hematol.
Volume: -
Issue: 2
Pages: 208-212
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A nationwide survey of hypoplastic myelodysplastic syndrome (a multicenter retrospective study)2017
Author(s)
Kobayashi T, Nannya Y, Ichikawa M, Oritani K, Kanakura Y, Tomita A, Kiyoi H, Kobune M, Kato J, Kawabata H, Shindo M, Torimoto Y, Yonemura Y, Tohyama K, et al
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Journal Title
American Journal of Hematology
Volume: 92
Issue: 12
Pages: 1324-1332
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Phase II Study of the Efficacy and Safety of Pembrolizumab for Relapsed/Refractory Classic Hodgkin Lymphoma.2017
Author(s)
Chen R, Zinzani PL, Fanale MA, Armand P, Johnson NA, Brice P, Radford J, Ribrag V, Molin D, Vassilakopoulos TP, Tomita A, von Tresckow B, Shipp MA, Zhang Y, Ricart AD, Balakumaran A, Moskowitz CH; KEYNOTE-087.
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Journal Title
J Clin Oncol.
Volume: 92(12)
Issue: 19
Pages: 1324-1332
DOI
Related Report
Peer Reviewed
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[Journal Article] Emetine elicits apoptosis of intractable B-cell lymphoma cells with MYC rearrangement through inhibition of glycolytic metabolism.2017
Author(s)
Aoki T, Shimada K, Sakamoto A, Sugimoto K, Morishita T, Kojima Y, Shimada S, Kato S, Iriyama C, Kuno S, Harada Y, Tomita A, Hayakawa F, Kiyoi H.
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Journal Title
Oncotarget.
Volume: 8
Issue: 8
Pages: 13085-13098
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Japanese phase II study of rituximab maintenance for untreated indolent B-cell non-Hodgkin lymphoma with high tumor burden.2016
Author(s)
Igarashi T, Ogura M, Itoh K, Taniwaki M, Ando K, Kuroda Y, Yamamoto K, Uike N, Tomita A, Nagai H, Kurosawa M, Mori S, Nawano S, Terauchi T, Ohashi Y, Tobinai K.
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Journal Title
Int J Hematol.
Volume: 104
Pages: 700-708
Related Report
Peer Reviewed / Open Access
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[Journal Article] Development and analysis of patient derived xenograft mouse models in intravascular large B-cell lymphoma.2016
Author(s)
Kazuyuki Shimada, Satoko Shimada , Keiki Sugimoto , Masahiro Nakatochi , Miyuki Katayama , Akihiro Hirakawa , Toby Hocking , Ichiro Takeuchi , Takashi Tokunaga , Yusuke Takagi , Akihiko Sakamoto , Tomohiro Aoki , Tomoki Naoe , Shigeo Nakamura , Fumihiko Hayakawa , Masao Seto , Akihiro Tomita , Hitoshi Kiyoi.
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Journal Title
Leukemia
Volume: in press
Related Report
Peer Reviewed
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[Presentation] Detection of Recurrent Genetic Mutations in Intravascular Large B-Cell Lymphoma By Comprehensive Genetic Analyses Using Peripheral Blood Circulating Cell-Free DNA.2017
Author(s)
Akihiro Tomita, Kazuyuki Shimada, Kenichi Yoshida, Yasuhiro Suzuki, Chisako Iriyama, Masashi Sanada, Masaaki Yuge, Yusuke Takagi, Shigeru Kusumoto, Yasufumi Masaki, Takahiko Ito, Yuichiro Inagaki, Akinao Okamoto, Yuichi Shiraishi, Kenichi Chiba, Hiroko Tanaka, Satoru Miyano, Seishi Ogawa and Hitoshi Kiyoi.
Organizer
59th Annual meeting od American Society of Hematology
Related Report
Int'l Joint Research
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[Presentation] Emetine Elicits Apoptosis of Intractable B-Cell Lymphoma Cells with MYC Rearrangement through Inhibition of Glycolytic Metabolism2016
Author(s)
Tomohiro Aoki, Kazuyuki Shimada, Akihiko Sakamoto, Keiki Sugimoto, Takanobu Morishita, Yuki Kojima, Satoko Shimada, Seiichi Kato, Chisako Iriyama, Shunsuke Kuno, Yasuhiko Harada, Shigeo Nakamura, Akihiro Tomita, Fumihiko Hayakawa and Hitoshi Kiyoi
Organizer
American Society of Hematology, 58th Annual Meeting & Exposition
Place of Presentation
San Diego Convention Center, San Diego, CA, USA,
Year and Date
2016-12-04
Related Report
Int'l Joint Research
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[Presentation] Utilization of Peripheral Blood Cell-Free DNA in Myelodysplastic Syndromes: Clinical and Molecular Characteristics and Utilization for Genetic Analyses Using Conventional and Next-Generation Strategies.2015
Author(s)
Akihiro Tomita, Yasuhiro Suzuki, Fumika Nakamura, Chisako Iriyama, Mizuho Shirahata-Adachi, Kazuyuki Shimada, Akimi Akashi, Yuichi Ishikawa, Norio Kaneda, Hitoshi Kiyoi.
Organizer
The American Society of Hematology, 57th Annual Meeting.
Place of Presentation
Orlando, FL, USA
Year and Date
2015-12-06
Related Report
Int'l Joint Research
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