Investigation of mechanisms underlying the development of lymphoma and myeloma using a high-throughput mouse model system
Project/Area Number |
15K09492
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Hematology
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Research Institution | Aichi Medical University (2016-2017) Aichi Cancer Center Research Institute (2015) |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | 悪性リンパ腫 / マウスモデル / レトロウイルス / 遺伝子導入 / 遺伝子変異 / CARD11 / BCL6 |
Outline of Final Research Achievements |
We have investigated causative roles of given genes or mutated genes in the development of malignant lymphoma and multiple myeloma using our high-throughput mouse model system, in which germinal center B cells (normal counterparts of many lymphomas) were induced in vitro, infected with retroviruses for gene of interest, and transplanted into mice. Results showed that a combination of CARD11 mutant and BCL6 or a combination of Myc and Bcl2 was sufficient for the development of lymphoma. Our models may provide a platform for the development of therapy of these lymphomas known as being associated with poor clinical outcome. Although we were unable to develop a mouse myeloma model within the assigned period of time, we have developed a Cre-LoxP-mediated gene expression system employing retrovirus vector, and thus will make an effort to accomplish the aims.
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] STX11 functions as a novel tumor suppressor gene in peripheral T-cell lymphomas.2015
Author(s)
Yoshida N, Tsuzuki S, Karube K, Takahara T, Suguro M, Miyoshi H, Nishikori M, Shimoyama M, Tsukasaki K, Ohshima K, Seto M.
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Journal Title
Cancer Science
Volume: 106
Issue: 10
Pages: 1455-62
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] The rs3779620 in the PBK gene is closely associated with myeloma cell growth2017
Author(s)
Ota Akinobu, Karnan Sivasundaram, Md Wahiduzzaman, Mizuno Shohei, Uchino Kaori, Kanasugi Jo, Horio Tomohiro, Murakami Satsuki, Suzuki Susumu, Tsuzuki Shinobu, Konishi Hiroyuki, Ueda Ryuzo, Hosokawa Yoshitaka, Takami Akiyoshi
Organizer
第79会 日本血液学会学術集会
Related Report
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[Presentation] High expression of MEF2D-fusion protein by escaping from the regulation by microRNA2017
Author(s)
Hirano Daiki, Hayakawa Fumihiko, Yasuda Takahiko, Yamamoto Hideyuki, Kojima Yuki, Morishita Takanobu, Imoto Naoto, Tsuzuki Shinobu, Mano Hiroyuki, Naoe Tomoki, Kiyoi Hitoshi
Organizer
第79会 日本血液学会学術集会
Related Report
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