| Project/Area Number |
15K09526
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Collagenous pathology/Allergology
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| Research Institution | Kobe University |
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Principal Investigator |
Nakamachi Yuji 神戸大学, 医学部附属病院, 臨床検査技師 (80379429)
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| Co-Investigator(Kenkyū-buntansha) |
河野 誠司 神戸大学, 医学部附属病院, 特命教授 (20351512)
三枝 淳 神戸大学, 医学部附属病院, 講師 (20514970)
笠木 伸平 神戸大学, 医学(系)研究科(研究院), その他 (80457051)
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| Co-Investigator(Renkei-kenkyūsha) |
SAEGUSA JUN 神戸大学, 医学部附属病院, 講師 (20514970)
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| Research Collaborator |
KASAGI SHINPEI 神戸大学, 医学部附属病院, 助教 (80457051)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | リウマチ学 / エクソソーム / 関節リウマチ / マイクロRNA |
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| Outline of Final Research Achievements |
We have reported that miR-124 inhibits the progression of adjuvant-induced arthritis rats by the ankle injection. In this study we investigated whether systemic administration of miR-124-containig exosomes could ameliorate arthritis of mouse collagen induced arthritis (CIA) model. Exosomes carrying miR-124 were separated from culture supernatants of a rheumatoid arthritis synovium cell line and mouse adipose-derived stem cell line which were transfected with miR-124. A single administration of 10μg miR-124-containing exosomes, or multiple administration (three times) of 5 μg exosomes did not significantly inhibit the progression of arthritis in CIA.
It was considered that further qualitative improvement would be necessary to efficiently deliver micro RNA to arthritic joints.
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