NETs treated monocytes in immune system

• Project/Area Number: 15K09545
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Collagenous pathology/Allergology
• Research Institution: Hokkaido University
• Principal Investigator: Bohgaki Toshiyuki 北海道大学, 医学研究院, 助教 (50431367)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 自然免疫

Outline of Final Research Achievements

Human healthy CD14+ cells treated with neutrophil extra cellular traps (NETs) could differentiate into CD14+CD1a-CD11c+HLA-DR+ cells assessed by flow cytometry. Expression levels of type-I interferon mRNA including interferon-alpha and interferon-beta were increased in these cells quantified by real-time PCR. On the other hand, expression levels of interferon-gamma, type-II interferon, mRNA were not significant increased. These expression levels could not be confirmed at the protein levels by western blot or flow cytometry because of its low expression.

Research Products

• [Journal Article] Interferon-inducible Mx1 protein is highly expressed in renal tissues from treatment-naiive lupus nephritis, but not in those under immunosuppressive treatment. (2017)
  • Author(s): Shimizu Y, Yasuda S, Kimura T, Nishio S, Kono M, Ohmura K, Shimamura S, Kono M, Fujieda Y, Kato M, Oku K, Bohgaki T, Fukasawa Y, Tanaka S, Atsumi T
  • Journal Title: Modern Rheumatology Volume: Epub ahead of print Issue: 4 Pages: 1-9
  • DOI: 10.1080/14397595.2017.1404711
  • Peer Reviewed
• [Journal Article] Complement and thrombosis in the antiphospholipid syndrome. (2016)
  • Author(s): Oku K, Nakamura H, Kono M, Ohmura K, Kato M, Bohgaki T, Horita T, Yasuda S, Amengual O, Atsumi T.
  • Journal Title: Autoimmun Rev Volume: 15(10) Issue: 10 Pages: 1001-1004
  • DOI: 10.1016/j.autrev.2016.07.020
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ribophorin II is involved in the tissue factor expression mediated by phosphatidylserine-dependent antiprothrombin antibody on monocytes (2016)
  • Author(s): Fujieda Y., Amengual O., Matsumoto M., Kuroki K., Takahashi H., Kono M., Kurita Y., Otomo K., Kato M., Oku K., Bohgaki T., Horita T., Yasuda S., Maenaka K., Hatakeyama S., Nakayama K.I. and Atsumi T. et al.
  • Journal Title: Rheumatology Volume: 印刷中 Issue: 6 Pages: 1117-1126
  • DOI: 10.1093/rheumatology/kew005
  • Peer Reviewed / Open Access
• [Journal Article] Ribophorin II is involved in the tissue factor expression mediated by phosphatidylserine-dependent antiprothrombin antibody on monocytes. (2016)
  • Author(s): Fujieda Y, Amengual O, Kono M, Kurita T, Otomo K, Kato M, Oku K, Bohgaki T, Horita T, Yasuda Y, Kuroki K, Maenaka K, Matsumoto M, Hatakeyama S, Atsumi T.
  • Journal Title: Rheumatology (Oxford). Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Ras guanine nucleotide-releasing protein 4 is aberrantly expressed in the fibroblast-like synoviocytes of patients with rheumatoid arthritis and controls their proliferation. (2015)
  • Author(s): Kono M, Yasuda S, Stevens RL, Koide H, Kurita T, Shimizu Y, Kanetsuka Y, Oku K, Bohgaki T, Amengual O, Horita T, Shimizu T, Majima T, Koike T, Atsumi T.
  • Journal Title: Arthritis Rheumatol Volume: 67 Issue: 2 Pages: 396-407
  • DOI: 10.1002/art.38924
  • Peer Reviewed / Open Access
• [Journal Article] An independent validation of the global anti-phospholipid syndrome score in Japanese cohort of patients with antoimmune diseases (2015)
  • Author(s): Oku K, Amengual O, Bohgaki T, Horita T, Yasuda S, Atsumi T
  • Journal Title: Lupus Volume: 24 Issue: 7 Pages: 774-775
  • DOI: 10.1177/0961203314561284
  • Peer Reviewed / Open Access
• [Presentation] Induction of type-I interferon mRNA in human CD14+ cells treated with neutrophil extracellular traps (2016)
  • Author(s): Toshiyuki Bohgaki, et al
  • Organizer: 10th European Lupus Meeting
  • Place of Presentation: Venice, Italy
  • Year and Date: 2016-10-05
  • Int'l Joint Research
• [Presentation] Ectopic expression of RasGRP4 plays a critical role in inflammatory arthritis via phosphorylation of Erk and p38-MAPK. (2015)
  • Author(s): Shimamura S, Yasuda S, Shimizu Y, Kono M, Kato M, Bohgaki T, Horita T, Atsumi T.
  • Organizer: 日本免疫学会総会学術集会
  • Place of Presentation: 札幌コンベンションセンター(北海道札幌市)
  • Year and Date: 2015-11-18
• [Presentation] フルバスタチンは抗リン脂質抗体症候群においてHLA class IIによるb2グリコプロテインIの抗原提示を抑制する (2015)
  • Author(s): 渡邊 俊之、奥 健志、オルガ・アメングアル、久田 諒、大村 一将、志田 玄貴、清水 裕香、加藤 将、坊垣 暁之、堀田 哲也、保田 晋助、石津 明洋、荒瀬 尚、渥美 達也
  • Organizer: 日本臨床免疫学会総会
  • Place of Presentation: 神戸国際会議場(兵庫県神戸市)
  • Year and Date: 2015-10-23
• [Presentation] Predictive markers for thrombotic events in autoimmune diseases: comparison of Antiphospholipid Score (aPL-S) and Global Anti-phospholipid syndrome score (GAPSS). (2015)
  • Author(s): Oku K, Amengual O, Hisada R, Oomura K, Nakagawa I, Watanabe T, Bohgaki T, Horita T, Yasuda S, Atsumi T.
  • Organizer: International Society on Thrombosis and Haematosis Congress
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2015-06-20
  • Int'l Joint Research
• [Presentation] Usefulness of whole-body MRI for the evaluation of patients with rheumatoid arthritis on biological agents. (2015)
  • Author(s): Kono M, Yasuda S, Ohmura K, Shimamura S, Noguchi A, Nakagawa I, Shida H, Watanabe T, Shimizu Y, Oku K, Bohgaki T, Amengual O, Horita T, Kamishima T, Atsumi T.
  • Organizer: 日本リウマチ学会学術集会
  • Place of Presentation: 名古屋国際会議場(愛知県名古屋市)
  • Year and Date: 2015-04-23
• [Book] 細胞表面分子に対する生物学的製剤と免疫学 (2015)
  • Author(s): 坊垣 暁之、渥美 達也
  • Publisher: 医学のあゆみ
• [Book] BAFFをターゲットとした治療の現状 (2015)
  • Author(s): 坊垣 暁之
  • Publisher: 分子リウマチ治療