Regulations of inflammatory responses induced by Chlamydia

• Project/Area Number: 15K09561
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Hokkaido University
• Principal Investigator: Matsuo Junji 北海道大学, 保健科学研究院, 講師 (50359486)
• Co-Investigator(Kenkyū-buntansha): 中村 眞二 順天堂大学, 医学(系)研究科(研究院), 助教 (40207882) ; 山口 博之 北海道大学, 保健科学研究院, 教授 (40221650)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: クラミジア / 炎症 / サイトカイン / アポトーシス / 宿主炎症応答 / miRNA / 炎症性サイトカイン

Outline of Final Research Achievements

To understand host inflammatory responses induced by Chlamydia, we tried to identify a novel receptor of host cells and chlamydial factors, which are conserved in the Chlamydiales. As a result, a non-TLR receptor and a heat-labile chlamydial factor may be involved in host inflammatory responses. In addition, the responses were enhanced in low oxygen environments. On the other hands, host caspase-3 dynamics was revealed using a cyclic fire luciferase probe. We also showed that miRNA regulation may be involved in the host inflammatory responses induced by Chlamydia.

Research Products

• [Journal Article] Acanthamoeba containing endosymbiotic chlamydia isolated from hospital environments and its potential role in inflammatory exacerbation. (2016)
  • Author(s): Fukumoto T, Matsuo J, Okubo T, Nakamura S, Miyamoto K, Oka K, Takahashi M, Akizawa K, Shibuya H, Shimizu C, Yamaguchi H.
  • Journal Title: BMC Microbiol. Volume: 16 Issue: 1 Pages: 292-292
  • DOI: 10.1186/s12866-016-0906-1
  • NAID: 120005905631
  • Peer Reviewed / Open Access
• [Presentation] Chlamydia trachomatisは感染後期に宿主細胞のカスパーゼ3を活性化する (2018)
  • Author(s): 松尾淳司，大久保寅彦，中村眞二，山口博之
  • Organizer: 第91回日本細菌学会総会（福岡市）
• [Presentation] ヒトの移動に伴い院内に持ち込まれるアメーバ共生細菌とヒトへのリスク． (2017)
  • Author(s): 松尾淳司，中村眞二，大久保寅彦，山口博之
  • Organizer: 第90回日本細菌学会総会
  • Place of Presentation: 仙台国際センター（宮城）
  • Year and Date: 2017-03-19
  • Invited
• [Presentation] カスパーゼ3プローブ発現細胞を用いたクラミジア感染宿主細胞内のアポトーシス制御機構の探索 (2017)
  • Author(s): 松尾淳司，芳賀早苗，大久保寅彦，中村眞二，小沢岳昌，尾崎倫孝，山口博之
  • Organizer: ConBio2017(神戸市)
• [Presentation] Amoebal endosymbiont Protochamydia isolated from a hospital can induce IL-8 in human immortal HEp-2 cells. (2016)
  • Author(s): Matsuo J., Fukumoto T., Okubo T., Nakamura S., Akizawa K., Shibuya H., Shimizu C., Yamaguchi H.
  • Organizer: ASM Microbe 2016.
  • Place of Presentation: Boston (USA)
  • Year and Date: 2016-06-16
  • Int'l Joint Research