| Project/Area Number |
15K09635
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Osama Woman's and Children's Hospital |
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Principal Investigator |
Yamazaki Miwa 地方独立行政法人大阪府立病院機構大阪母子医療センター(研究所), 環境影響部門, 研究員(移行) (50455549)
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| Co-Investigator(Renkei-kenkyūsha) |
MICHIGAMI Toshimi 地方独立行政法人大阪府立病院機構, 大阪母子医療センター(研究所)・環境影響部門, 部長 (00301804)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | リン / Pit-1 / Pit-2 |
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| Outline of Final Research Achievements |
As osteoblasts mature, they acquire the expression of multiple molecules involved in phosphate (Pi) metabolism. Osteoblasts/osteocytes may sense and respond to alterations in Pi availability in their microenvironment. However, the involvement of type III sodium/phosphate co-transporters, Pit-1 and Pit-2, in Pi handling in osteoblasts/osteocytes is unclear. In the current study, we have investigated the role of Pit-1 and Pit-2 in the responsiveness of osteoblasts to Pi by deleting Pit-1 and Pit-2 from MC3T3-E1 cells using CRISPR/Cas9 systems. Deletion of Pit-1 in osteoblastic cells affected the response to increased extracellular Pi. Although Pit-2 may play a role to mediate a long-term effect of high Pi in osteoblastic cells, it appears to be dispensable for responding to acute Pi elevation. In conclusion, Pit-1 and Pit-2 might have different roles in osteoblasts.
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