| Project/Area Number |
15K09649
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Mie University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
平山 雅浩 三重大学, 医学系研究科, 教授 (90293795)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 小児白血病 / 白血病微小環境 / N-カドヘリン分子 / N-カドヘリン |
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| Outline of Final Research Achievements |
We investigated whether N-cadherin, one of the adhesion molecules, could play pivotal roles in resistance against anti-leukemic drugs in childhood leukemia. We showed the sensitivity for dexamethasone, not L-asparaginase, Vincristine or Methotrexate, in 697 leukemia cell line with the highly expression of N-cadherin using the neutralizing antibody (GC-4) was increased in cytotoxic assay. Moreover, we developed N-cadherin knock-down bone marrow stroma cell (BMSC) lines to investigate this specific mechanism in bone marrow microenvironment niche model. In next step, we will examine the influence for dexamethasone in the co-culture system using 697 and BMSC with various expression of N-cadherin and to study the molecular mechanism.
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