identification for leukemic stem cells using microRNA sensor vector for developing new therapy
| Project/Area Number |
15K09652
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | microRNA / 白血病 / 小児急性白血病 / 急性リンパ性白血病 / 白血病幹細胞 |
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| Outline of Final Research Achievements |
Recently microRNAs are investigated as a therapeutic marker or a target for hematologic malignancies. Based on the results of miR analysis of primary pediatric acute lymphoblastic leukemia (ALL), we picked up several target miRs including miR-146a and investigated its activities in various ALL cell lines using a miR sensing vector and a miR knock down vector. Mir-146a is generally upregulated and differentially expressed in most cell lines. Knocking down of this miR resulted in 30-50% reduction of colony formation. Underlying molecular mechanism is under investigation by RNA-seq.
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Report
(4 results)
Research Products
(3 results)
