The role of the heterotrimeric G protein, Galpha12/13 in differentiation and proliferation of smooth muscle cells.

• Project/Area Number: 15K09683
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Nippon Medical School (2017); The University of Tokyo (2015-2016)
• Principal Investigator: Suzuki Nobuchika 日本医科大学, 大学院医学研究科, 研究生 (90247007)
• Co-Investigator(Kenkyū-buntansha): 川村 猛 東京大学, アイソトープ総合センター, 准教授 (70306835) ; 小笹 徹 東京大学, 先端科学技術研究センター, 特任教授 (70202059)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 平滑筋分化 / 三量体G蛋白 / リン酸化 / 血管平滑筋細胞分化 / 三量体G蛋白質G12 / リン酸化修飾 / RH-RhoGEF / Gbeta / Ggamma / Gα12/13 / Rho活性化調節 / 平滑筋細胞分化 / Rho / G12 / TGFβ

Outline of Final Research Achievements

Losing a balance between differentiation and proliferation of smooth muscle cells causes the hereditary aortic aneurysms. Our study indicates that a heterotrimeric G protein, Galpha12 activates the pathway divergent from Galpha13.Phosphorylation of Galpha12 by TGFbeta stimulation may sustain the activation of the pathway.

Research Products

• [Int'l Joint Research] Thomas Jefferson University(米国)
• [Journal Article] Targeting gene expression to specific cells of kidney tubules in vivo, using adenoviral promoter fragments. (2017)
  • Author(s): Sumiyo Watanabe , Toru Ogasawara, Yoshifuru Tamura, Taku Saito, Toshiyuki Ikeda, Nobuchika Suzuki, Tatsuo Shimosawa, Shigeru Shibata, Ung-il Chung, Masaomi Nangaku, Shunya Uchida
  • Journal Title: PLoS One Volume: 12 Issue: 3 Pages: 1-14
  • DOI: 10.1371/journal.pone.0168638
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Mitotic-dependent phosphorylation of leukemia-associated RhoGEF (LARG) by Cdk1 (2015)
  • Author(s): Helms, M. C. Grabocka, E. Martz, M. K. Fischer, C. C. Suzuki, N. Wedegaertner, P. B.
  • Journal Title: Cell Signal Volume: 28 Issue: 1 Pages: 43-52
  • DOI: 10.1016/j.cellsig.2015.10.004
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant